新城疫病毒ClassⅠ强毒株HN单克隆抗体的研制及免疫学反应  

Preparation of monoclonal antibodies against HN protein of ClassⅠ Newcastle disease virus virulent strain

作　　者：孙英杰[1] 陈鸿军[1] 宋翠萍[1] 于洋[1] 仇旭升[1] 于圣青[1] 丁铲[1] 

机构地区：[1]中国农业科学院上海兽医研究所,上海200241

出　　处：《细胞与分子免疫学杂志》2010年第5期464-466,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：中央公益性研究院所基础业务专项经费(2007JB09)

摘　　要：目的:制备抗新城疫病毒(NDV)ClassⅠ强毒分离株9a5b的单克隆抗体。方法:以9a5b尿囊液为免疫原,接种6周龄BALB/c小鼠,以血凝抑制(HI)和间接免疫荧光(IFA)检测所获得的mAb。结果:成功制备获得NDV血凝素(HN)特异性mAb4株。免疫特性鉴定表明:在这4株mAb中,3H7和3H9株仅具有IFA和HI效价,且呈现ClassI毒株特异性。结论:该抗体材料为NDVClassⅠ和Ⅱ毒株的血清学鉴定提供极大的便利工具,并为NDVHN功能及受体学研究奠定了基础。AIM：To prepare monoclonal antibodies （mAb） against HN protein of Class I Newcaslte disease virus.METHODS：Several 6-week-old mice were immunized with ClassⅠ 9a5b virulent strain.Hemagglutination inhibition （HI）,indirect immunofluorescence assay （IFA）,cell neutralization test,ELISA and Western blot analysis were used to characterize and identify these antibodies.RESULTS：Four hybridoma cell lines were successfully prepared,designated as mAb 2F5,2F12,3H7 and 3H9.Among them,only mAb 3H7 and 3H9 recognized HN protein of the ClassⅠ strain,specifically neutralizing NDV and inhibiting hemagglutination.CONCLUSION：mAb 3H7 and 3H9 could be of use in identification of Class I and Class II strains,and in functional studies of HN and cell receptors for NDV.

关 键 词：新城疫病毒 ClassⅠ 强毒株 HN 单克隆抗体 

分 类 号：R392.11[医药卫生—免疫学]