马齿苋多糖提取物对翅果油树愈伤组织诱导的影响  

Induction of Elaeagnus mollis Callus on Medium with Portulaca oleracea Polysaccharides

作　　者：杜贞[1] 穆楠[1] 武银玉[1] 闫桂琴[1] 

机构地区：[1]山西师范大学生命科学学院生物多样性研究所,山西临汾041004

出　　处：《西北植物学报》2010年第3期610-614,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30470296);山西省自然科学基金(20031090)资助

摘　　要：采用传统热浸提工艺,从马齿苋植株中提取多糖。以B1培养基为基础,通过添加0.15g/L的抗褐变剂Vc及不同浓度的马齿苋多糖,研究了其对翅果油树愈伤组织培养的抗褐变作用。结果显示:(1)马齿苋多糖对愈伤组织培养中的褐变有一定缓解作用。(2)经筛选,得到0.19g/L为最适的多糖添加物浓度,子叶愈伤组织产生生物量最多,抗褐变效果与Vc的作用相似,愈伤组织生命周期也明显缩短。Using B1 as basic medium,we analyzed the effect of Portulaca oleracea polysaccharides on callus induction of Elaeagnus mollis by adding 0.15 g/L Vc and six different concentrations of polysaccharides.The result indicated that doses of supplement avail to lighten browning.Among the six concentrations of polysaccharides extraction of Portulaca oleracea the most tissue biomass obtained on medium with 0.19 g/L polysaccharides.The effects of anti-browning were similar as with Vc（0.15 g/L）,and the callus growth circles were obviously reduced.

关 键 词：翅果油树 愈伤组织 马齿苋 多糖 褐变 

分 类 号：Q813.12[生物学—生物工程]