重组内皮抑素腺病毒联合卡铂抗小鼠肺癌的实验研究  

Antitumor Effect by Combination of Recombinant Endostatin Adenovirus with Carboplatin

作　　者：廖加群[1] 苏晓庆[1] 彭星辰[1] 石华山[1] 张海龙[1] 杨莉[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室,成都610041

出　　处：《四川大学学报（医学版）》2010年第3期386-389,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家863重点项目基金(2007AA021202)资助

摘　　要：目的将重组内皮抑素腺病毒(Ad-E)和化疗药物卡铂联合治疗小鼠Lewis肺癌,观察其对肿瘤的抑制作用以探索其在肺癌治疗中的可行性。方法建立小鼠LL/2肺癌模型,将荷瘤小鼠随机分为5组:生理盐水(NS)组,空载腺病毒(Ad-null)组,卡铂组,Ad-E组,Ad-E+卡铂组;观察肿瘤体积、瘤重、小鼠生存期、肿瘤血管生成、肿瘤细胞凋亡及治疗毒副反应。结果与Ad-E或卡铂单药组相比,联合治疗组的肿瘤生长减慢,瘤重减轻,小鼠生存期延长,肿瘤组织微血管密度减少,肿瘤细胞凋亡增加,差异均有统计学意义(P<0.05)。各治疗组小鼠均未出现明显毒副反应。结论以腺病毒为载体的抗血管基因治疗联合化疗有较好的抑制小鼠Lewis肺癌效应,且无明显毒副作用。Objective To investigate the antitumor effect of recombinant Endostatin adenovirus（Ad-E） with carboplatin in mice with Lewis lung cancer.Methods C57BL/6 mice bearing Lewis lung cancer received Ad-E and carboplatin respectively or in combination.The tumor volume,tumor net weight,survival time and side effects were observed.Histological analysis was conducted to assess microvessel density（MVD） within tumor tissue and apoptotic cells were identified using the TUNEL assay.Results Compared with the control groups,the combination treatment significantly suppressed the tumor growth and prolonged survival time of the mice without obvious side-effects.The histological analysis revealed the combination treatment led to a decreased MVD and increased percent apoptosis in LL/2 tumors.Conclusion Our studies indicate that a combination of Ad-E with carboplatin can present better antitumor effects without obvious side-effect.

关 键 词：内皮抑素 卡铂 基因治疗 肺癌 

分 类 号：R73-362[医药卫生—肿瘤]