瘦素对缺血缺氧损伤脑星形胶质细胞凋亡的影响  

Effects of Leptin on Apoptosis of Rat Cerebral Astrocytes with Ischemia/Hypoxia Injury

作　　者：司艺玲[1] 张金英[1] 郝秀华[1] 邓子辉[1] 薛辉[1] 颜光涛[1] 

机构地区：[1]解放军总医院基础医学研究所生物化学研究室,北京100853

出　　处：《四川大学学报（医学版）》2010年第3期403-407,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30670821)资助

摘　　要：目的研究瘦素对缺血缺氧/复氧损伤星形胶质细胞凋亡的影响及其作用机制。方法制作缺血缺氧大鼠脑皮质星形胶质细胞损伤模型,MTT法检测星形胶质细胞活力;采用Annexin V-FITC试剂盒检测星形胶质细胞凋亡;RT-PCR和Western blot方法检测凋亡相关基因bcl-2、bax、caspase-3的表达。结果与缺血缺氧损伤组比较,瘦素干预组星形胶质细胞存活率升高(P<0.01)、凋亡率下降(P<0.01);抑凋亡基因bcl-2 mRNA和蛋白表达水平上调(P<0.01),促凋亡基因bax、caspase-3 mRNA和蛋白表达水平下调(P<0.01),呈剂量依赖性。结论瘦素能够使凋亡相关基因bcl-2表达上调、bax和caspase-3表达下调,从而抑制缺血缺氧损伤星形胶质细胞发生凋亡。Objective To investigate the effect of leptin on apoptosis of rat cerebral astrocytes with ischemia/hypoxia injury and its mechanism.Methods The cerebral astrocytes with ischemic/hypoxia injury were induced in neonatal SD rats.The cellular viability of injury of astrocytes was detected by MTT assay.The apoptosis of astrocyte were detected with Annexin V-FITC kit.The effect of leptin on the expression of apoptosis factor bcl-2,bax,caspase-3 was detected by RT-PCR and Western blot.Results Compared with the ischemia group,the cellular viability of leptin intervention group increased significantly（P〈0.01）,while the astrocytes apoptosis of leptin intervention group decreased significantly（P〈0.01）.The mRNA and protein expression level of anti-apoptosis factor bcl-2 in leptin intervention group was much higher than that of ischemia group（P〈0.01）,whereas the mRNA and protein expression of bax and caspase-3 was much lower than that of ischemia group（P〈0.01）.Conclusion Leptin could significantly decrease the apoptosis of astrocytes with ischemia/hypoxia injury,and it is relevant to the increase of bcl-2 expression and the decrease of bax caspase-3 expression level.

关 键 词：瘦素 星形胶质细胞 缺血 缺氧 凋亡 

分 类 号：R741[医药卫生—神经病学与精神病学]