吗啡预处理对兔缺氧/复氧损伤肝脏组织的保护作用  

Protective Effect of Morphine Hypoxic Preconditioning on Liver Against Acute Hypoxic-reoxygenation Injury in Rabbits

作　　者：江晓琴[1] 倪娟[1] 杨沛[2] 黄蔚[1] 罗金凤[1] 李华凤[1] 

机构地区：[1]四川大学华西第二医院麻醉科,成都610041 [2]四川大学华西第二医院妇产科,成都610041

出　　处：《四川大学学报（医学版）》2010年第3期412-414,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30672025)资助

摘　　要：目的探讨吗啡预处理对兔缺氧/复氧损伤肝脏组织的保护作用及其机制。方法雄性新西兰大白兔30只随机分成正常对照组(N组)、缺氧复氧组(H/R组)和吗啡预处理组(MO+H/R组)。H/R组和MO+H/R组分别静脉注射生理盐水5 mL和吗啡3 mg/kg后,吸入8%O23 h,空气复氧3 h。比较各组肝脏组织凋亡指数(AI)、超氧化物歧化酶(SOD)水平和肝脏组织病理学改变。结果与N组比,H/R组肝脏组织AI增加〔(0.43±0.13)%vs.(9.50±1.00)%,P<0.05〕,而MO+H/R组〔(2.20±0.49)%〕比H/R组肝脏组织AI降低(P<0.05)。与H/R组相比,吗啡预处理可增强肝脏组织中SOD的表达(P<0.05)。结论吗啡预处理可增强肝脏组织SOD的活力,减轻氧自由基引起的细胞凋亡,进而对缺氧/复氧损伤肝脏发挥保护作用。Objective To investigate the protective effect of morphine hypoxic preconditioning on hepatic hypoxic/reoxygenation injury in rabbits and the mechanisms involved.Methods Hypoxic/reoxygenation injury was induced with inhalation of 8% O2 for 3 hours followed by air for 3 hours.Thirty male white New Zealand rabbits were randomly divided into 3 groups： normal control group（N group）,hypoxic/reoxgenation group（H/R group） and morphine hypoxic preconditioning group（MO＋H/R group）.Animals in the H/R and MO＋H/R groups received 5 mL of saline or 3 mg/kg of morphine respectively before the induction of hypoxic injury.Hepatic apoptosis was determined quantitatively by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling（TUNEL） methods.The activity of superoxide dismulase（SOD） in liver tissues was measured at the end of reoxgygenation.Results A large number of TUNEL positive cells（（9.50±1.00）%） were observed in hepatic tissues of rabbits in the H/R group.The administration of morphine exerted a significant anti-apoptotic effect,as evidenced by reduced TUNEL-positive staining（（2.20±0.40）%,P〈0.05 vs.H/R group）.Compared with the H/R group,treatment with morphine increased SOD activity significantly（（85.57±19.37） vs.（48.35±15.84）,P〈0.05））.Conclusion Morphine hypoxic preconditioning can protect rabbits against acute hepatic hypoxic/reoxygenation injury by increasing SOD activity and reducing hepatic apoptosis.

关 键 词：吗啡预处理 缺氧/复氧损伤 细胞凋亡 

分 类 号：R363[医药卫生—病理学]