乳腺癌患者CD4^+CD25^(high)CCR6^+调节性T细胞的变化  

The Change of CD4^+CD25^(high)CCR6^+ Regulatory T Cells in Breast Cancer Patients

作　　者：徐林[1] 周涯[2] 肖代敏[3] 覃明[1] 罗军敏[1] 汤贤英[1] 

机构地区：[1]贵州省遵义医学院免疫学教研室,遵义563003 [2]贵州省遵义医学院医学物理学教研室,遵义563003 [3]贵州省遵义医学院附属医院检验科,遵义563003

出　　处：《四川大学学报（医学版）》2010年第3期415-419,共5页Journal of Sichuan University(Medical Sciences)

基　　金：遵义医学院博士科研启动基金(F-329);贵州省科学技术基金(C491)资助

摘　　要：目的检测临床乳腺癌患者肿瘤浸润淋巴细胞(TILs)和外周血单个核细胞(PBMCs)中CD4+CD25highCCR6+调节性T细胞的变化,并探讨其意义。方法分离22例乳腺癌患者PBMCs和TILs,用流式细胞仪检测CD4+CD25highCCR6+调节性T细胞的比例,同时检测其Foxp3的表达;进一步用流式细胞仪检测CD4+CD25highCCR6+调节性T细胞记忆分子CD45RO、CD44和CD62L的表达水平;用3H-TdR增殖实验观察CD4+CD25highCCR6+调节性T细胞对CD4+CD25-T细胞增殖的抑制作用。结果乳腺癌患者PBMCs和TILs中CD4+CD25highCCR6+调节性T细胞的比例明显增加,并在肿瘤局部存在显著的富集;CD4+CD25highCCR6+调节性T细胞高表达Foxp3,在体外能有效抑制CD4+CD25-T细胞的增殖,并高表达记忆分子CD45RO、CD44,低表达CD62L,显示了效应/记忆样表型。结论 CD4+CD25highCCR6+调节性T细胞在乳腺癌患者肿瘤局部有明显富集,这可能是肿瘤长期免疫逃逸的重要原因。Objective To detect the proportion of CD4^＋CD25^highCCR6^＋ regulatory T cells in peripheral blood mononuclear cells and tumor infiltration lymphocytes from breast cancer patients and explore its significance.Methods Lymphocytes isolated from blood and tumor mass of breast cancer patients were analyzed for the proportion of CD4^＋CD25^highCCR6^＋ regulatory T cells by flow cytometry.The expression of Foxp3 on CD4^＋CD25^highCCR6^＋ regulatory T cells,as well as CD45RO,CD44 and CD62L,were also analyzed.The effects of CD4^＋CD25^highCCR6^＋ regulatory T cells on the proliferation of CD4＋CD25-T cells also were detected by 3H-incorporation assay.Results Compared to the control,increased proportion of CD4^＋CD25^highCCR6^＋ regulatory T cells was observed in PBMCs and TILs from breast cancer patients.Moreover,CD4^＋CD25^highCCR6^＋ regulatory T cells,expressing high level of Foxp3,displayed effector memory phenotype determined by high level expression of CD45RO,CD44 and low level of CD62L.In addition,CD4^＋CD25^highCCR6^＋ regulatory T cells could inhibit the proliferation of CD4＋CD25-T cells in vitro.Conclusion The enrichment of CD4^＋CD25^highCCR6^＋ regulatory T cells in tumor mass in breast cancer patients,which might be close related to long term immunoescape of tumor.

关 键 词：CD4+CD25highCCR6+调节性T细胞 乳腺癌 肿瘤浸润淋巴细胞 

分 类 号：R737.9[医药卫生—肿瘤]