CXCR4、VEGF在血管母细胞瘤血管生成中的表达及意义  

Expression of CXCR4 and VEGF in Hemangioblastomas of the Central Nervous System and Its Relation to Tumor Angiogenesis

作　　者：王涛[1] 惠旭辉[2] 周良学[2] 鞠延[2] 戎宏涛[2] 张乔[2] 

机构地区：[1]四川大学华西第二医院儿科,成都610041 [2]四川大学华西医院神经外科,成都610041

出　　处：《四川大学学报（医学版）》2010年第3期420-423,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨中枢神经系统血管母细胞瘤中基质细胞衍生因子-1(SDF-1)受体CXCR4和血管内皮生长因子(VEGF)的表达水平及其与肿瘤血管生成的相互作用及关系。方法通过免疫组化SP法检测40例中枢神经系统血管母细胞瘤CXCR4及VEGF的表达。以CD34单克隆抗体标记瘤组织血管内皮细胞并测定微血管密度(MVD)。结果 CXCR4、VEGF在血管母细胞瘤中阳性表达率分别为95%(38/40)、85%(34/40),其表达强度高于正常脑组织(P<0.001)。CXCR4主要定位于肿瘤基质细胞的细胞核,VEGF主要定位于肿瘤基质细胞和血管内皮细胞胞浆。CXCR4与VEGF的表达呈正相关(r=0.704,P<0.001)。MVD与CXCR4、VEGF的表达状态有关。VHL病相关性和散发性、囊性和实性血管母细胞瘤中CXCR4、VEGF和MVD的差异均无统计学意义(P>0.05)。结论 CXCR4及VEGF在血管母细胞瘤血管生成中有协同作用。Objective To evaluate the relationships of CXCR4（chemokine stromal cell-drived factor-1 receptor） and VEGF（vaseular endothelial growth factor） expression to tumor angiogenesis in hemangioblastomas of the central nervous system.Methods The protein expressions of CXCR4 and VEGF were detected by SP immunohistochemical staining in 40 hemangioblastomas.The endothelial cells of blood vessels within the tumor were labeled by CD34,then the microvessel density（MVD） was calculated.Results Total positive expression rates of CXCR4 and VEGF were 95%（38/40） and 85%（34/40） respectively.The expression rates of CXCR4 and VEGF were found positive obviously in hemangioblastomas compared with the normal cerebellar tissues（P0.01）.CXCR4 expression was located in the nucleus of tumor stromal cells.VEGF expression was located in the cytoplasm and membrane of tumor stromal cells and some endothelial cells.VEGF has significant positive correlation with the expression level of CXCR4（r=0.704,P〈0.001）.MVD also has significant positive correlation with the expression level of CXCR4 and VEGF（P〈0.001）.But there is no significant difference between the cystic and solid tumors,VHL disease and sporadic disease on the expression level of CXCR4,VEGF and MVD（P〉0.05）.Conclusion CXCR4 and VEGF may collaborate to induce angiogenesis in hemangioblastomas.

关 键 词：血管母细胞瘤 CXCR4 VEGF MVD 血管生成 

分 类 号：R739.41[医药卫生—肿瘤]