骆驼蓬种子凝集素粗品活性的初步研究  

Preliminary Study of the Activities of the Crude Lectin from Peganum harmala Seeds

作　　者：唐海淑[1] 宁雪飞[1] 陈亮亮[1] 殷晓丽[1] 罗晶晶[1] 马晓瑾[1] 孙素荣[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《西北植物学报》2010年第4期813-820,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：新疆高校科研计划重点项目(XJEDU2008I01);新疆生物资源基因工程重点实验室开放基金项目(XJDX0201-2009-03);“国家大学生创新性实验计划”(081075507)

摘　　要：采用硫酸铵分级沉淀法提取骆驼蓬种子凝集素粗品,并进行凝血活性和抗菌活性检测。凝血实验结果表明：（1）50%-60%硫酸铵饱和度下提取骆驼蓬种子的凝集素粗品对鸡红细胞的凝集活力最强,最低凝集浓度为0.6 mg/L,且其凝集素粗品的活力受温度、pH值、金属离子及糖溶液的影响。（2）不同硫酸铵饱和度沉淀获得的凝集素粗品对6种人体致病细菌及5种能引起水果腐烂和粮食霉变的真菌均有不同程度的抑制作用,其中50%-60%硫酸铵饱和度下提取的凝集素粗品抑菌活性最强,其浓度为1.071 mg/mL时对鲍曼不动杆菌和意大利青霉菌的抑菌环半径分别可达8.1 mm和4.9 mm。The crude proteins of Peganum harmala L.seeds were extracted by the stepwise addition of saturated ammonium sulfate and their hemagglutination and antimicrobial activity were detected.The results of hemagglutination assay indicated the crude lectin fractionated by 50%～60% saturation of ammonium sulfate had the highest hemagglutination activity for the red bleed cells of chicken,the lowest concentration of hemagglutination is 0.6 mg/L.The data showed that the temperature,pH,metal ion,and carbohydrate solution could affect the hemagglutination activity of the crude lectin.The results showed that the crude lectin by the different saturation of sulfate ammonium displayed different inhibition between 6 kinds of bacteria and 5 kinds of fungi,the former were able to cause human disease,and the latter were able to cause fruits rotted and food supplies gone moldy.The crude lectin obtained by 50%～60% saturation of ammonium sulfate had the highest inhibitory activity,the radii of antibacterial ring on Acinetobacter baumannii and Penicillium italicum were up to 8.1 mm and 4.9 mm respectively when the concentration of the crude lection was 1.071 mg/mL.It is suggested that the study of the activities of the crude lectin from P.harmala seeds could provide some scientific evidence for producing antimicrobial medical drugs and cultivating transgenic plants which have antifungal activity.

关 键 词：骆驼蓬 硫酸铵分级沉淀 凝集素粗品 凝血活性 抑菌活性 

分 类 号：Q949.96[生物学—植物学]