两个品种猴腿蹄盖蕨总黄酮含量的测定及抗氧化活性研究  

Study on Content and Antioxidation Effect of Total Flavonoids from Two Kinds of Athyrium Multidentatum(Doll.) Ching.

作　　者：刘冬梅[1] 盛继文[2] 李万忠[1] 张辘辘[1] 李少霞 

机构地区：[1]潍坊医学院药学教研室 [2]潍坊医学院化学教研室 [3]齐鲁制药有限公司,济南市250101

出　　处：《光谱实验室》2010年第3期803-808,共6页Chinese Journal of Spectroscopy Laboratory

摘　　要：初步建立两个品种猴腿蹄盖蕨不同部位总黄酮的HPLC指纹图谱,并测定总黄酮含量,研究其抗氧化活性。采用RP-HPLC法,建立不同部位总黄酮的HPLC指纹图谱;紫外分光光度法测定总黄酮含量及对HO.和O2-.的清除率。两个品种猴腿蹄盖蕨总黄酮含量均较高,HPLC指纹图谱显示地上部分总黄酮成分类似,根总黄酮成分有差异;不同部位总黄酮粗提物对HO.和O2-.均有清除作用。猴腿蹄盖蕨总黄酮具有较强的抗氧化活性,值得深入研究。The HPLC fingerprint,content and antioxidation effect of total flavonoids from two kinds of Athyrium multidentatum（Doll.） Ching.were studied.Fingerprints for different parts of total flavonoids were obtained by RP-HPLC.The content of total flavonoids and HO· and O-·2 scavenging capacities were determined by ultraviolet spectrophotometry.The content of total flavonoids was high.Fingerprints of total flavonoids for overground parts of differrent Athyrium multidentatum（Doll.） Ching.were similar,and which for root were different.Total flavonoids from different parts have showed obvious scavenging effect on HO· and O-·2.The antioxidation effect of total flavonoids from different parts of two kinds of Athyrium multidentatum（Doll.） Ching.was obvious and deserved for further study.

关 键 词：猴腿蹄盖蕨 蹄盖蕨科 分光光度法 高效液相色谱 指纹图谱 总黄酮 抗氧化活性 

分 类 号：O657.32[理学—分析化学] O657.72[理学—化学]