肝癌中cyclin D1扩增与HBV感染及病理学的关系  

Cyclin D1 amplification is associated with HBV DNA integration and pathology in human hepatocellular carcinoma *

作　　者：王辉云[1] 严瑞琪[1] 龙江斌[1] 吴秋良[1] 

机构地区：[1]中山医科大学肿瘤防治中心

出　　处：《世界华人消化杂志》1999年第2期98-100,共3页World Chinese Journal of Digestology

基　　金：国家教委博士学科点专项科研基金;广东省自然科学基金

摘　　要：目的探讨癌基因ｃｙｃｌｉｎＤ１在肝癌发生发展中的作用以及与ＨＢＶ感染和临床病理之间的关系．方法取手术切除的肝细胞癌标本，用Ｓｏｕｔｈｅｒｎ杂交的方法对ｃｙｃｌｉｎＤ１和ＣＤＫ４癌基因以及ＨＢＶ的感染状态进行了检测，切片同时行病理检查．结果肝癌３９例中９例ｃｙｃｌｉｎＤ１存在２～３０倍的扩增（２３７％），其中１例检出重组杂交带；３７例中５例（１３５％）有ＣＤＫ４的扩增；ＨＢＶＤＮＡ在肝癌组织中检出的阳性率为９４７％，ＨＢＶＤＮＡ整合率为７４４％．相关分析发现，ｃｙｃｌｉｎＤ１的扩增与ＣＤＫ４扩增，ＨＢＶＤＮＡ整合，肿瘤的分化高低和肿块大小等之间有相关关系，相关系数ｒ分别为０５１，０３１，０３９和０５５，全部Ｐ＜００５．结论ｃｙｃｌｉｎＤ１的扩增与ＨＢＶＤＮＡ的整合密切相关，并在肝癌的恶化进展中起着重要作用．AIM To explore the amplification of cyclin D1 and its correlation to HBV infection and pathology during the development of human hepatocellular carcinoma (HCC). METHODS Forty specimens of fresh paired tumor and nontumor liver tissues from surgical resections were used for detection of genetic alterations of cyclin D1 , CD K 4 and HBV infection by the Southern hybridization. The samples were also examined histologically for pathological correlation with the genetic changes. RESULTS Two to thirty fold amplifications of cyclin D1 gene were detected in 9 of 39 HCC samples (23 7%) and a structural aberration of the gene was found in one of those samples with amplification. The amplifications of CD K 4 gene were found in 5 of 37 HCC cases. HBV DNA was detected in 37 of 39 HCC samples (94 9%) and the integration of HBV DNA in 29 of 39 HCC samples (74 4%). By correlation analysis, cyclin D1 amplification significantly correlated with amplification of CD K 4, the integration of HBV DNA, the differentiation degree and size of tumors. The correlation coefficients were 0 51, 0 31,0 39 and 0 55 respectively, P <0 05 for all. CONCLUSION The amplification of cyclin D1 is related to HBV DNA integration and plays an important role in the development and progression of HCC.

关 键 词：肝肿瘤 病理学 肝细胞 DNA HBV 

分 类 号：R735.702[医药卫生—肿瘤]