人IL-12重组逆转录病毒载体在HeLa细胞中的表达  

Construction of recombinant retroviral vector carrying IL-12 gene and identification of its expression in HeLa cells

作　　者：张巨峰[1,2] 陈艳娜[1] 张文峰[1] 王俊伟[1] 牟艳芳[1] 黄树林[1] 

机构地区：[1]广东药学院生命科学与生物制药学院,广东广州510006 [2]深圳北京大学香港科技大学医学中心生物医学研究所,广东深圳518036

出　　处：《现代生物医学进展》2010年第7期1205-1207,共3页Progress in Modern Biomedicine

基　　金：中国博士后科学基金(20080440778);广东药学院博士启动基金(43555023)

摘　　要：目的:包装携带人白细胞介素12(IL-12)的逆转录病毒,用于宫颈癌的治疗研究。方法:携带IL-12的逆转录病毒重组质粒pL35P40SN经PA317细胞包装,G418筛选,在NIH3T3细胞进行病毒滴度测定。然后用病毒感染人宫颈癌细胞HeLa。PCR、RT-PCR方法检测IL-12基因在HeLa中的整合和表达情况。结果:重组质粒pL35P40SN经PA317细胞包装后收获病毒上清,感染HeLa细胞,检测发现IL-12基因整合到细胞基因组DNA中,并且能有效的转录。结论:成功包装了携带IL-12基因的逆转录病毒,该病毒能有效感染HeLa细胞,并使携带的基因IL-12在细胞中表达,为今后IL-12基因治疗宫颈癌的研究奠定基础。Objective：To package the recombinant retrovirus carrying interleukin 12（IL-12） gene and to observe the expression of IL-12 in cervical carcinoma cell line HeLa.Methods：The retroviral vector pL35P40SN was transferred into retroviral producer cell line PA317 for packaging retrovirus and then the resultant retrovirus was applied to infect the HeLa cells.The integration of IL-12 gene into the HeLa cells genome was assayed by PCR.The expression of IL-12 gene was detected by RT-PCR.Results：The IL-12 gene was integrated into the genome of HeLa cells shown by results of PCR after retroviral infection.The expression of IL-12 gene was confirmed by RT-PCR.Conclusion：The recombinant retrovirus carrying IL-12 gene was packaged successfully and may be a potentially effective strategy for the treatment of cervical cancer.

关 键 词：IL-12基因 逆转录病毒 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤] Q75[医药卫生—临床医学]