大鼠肝硬化形成中气体信号分子一氧化氮、一氧化碳对硫化氢/胱硫醚-γ-裂解酶体系的影响  

Effect of nitric oxide and carbon monoxide on hydrogen sulfide/cystathionine-γ-lyase in rats with hepatic cirrhosis

作　　者：宋丽秀[1] 郑勇[1] 陈卫刚[1] 刘清华[1] 张宁[1] 刘维国[1] 李文娟[1] 

机构地区：[1]石河子大学医学院第一附属医院消化科,新疆石河子832002

出　　处：《现代生物医学进展》2010年第7期1213-1216,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30850004)

摘　　要：目的:研究气体信号分子一氧化氮(NO)、一氧化碳(CO)在大鼠肝硬化中对硫化氢(H2S)/胱硫酶-γ-裂解酶(CSE)体系的影响。方法:将SD大鼠随机分为3组:正常对照组(NC组)、肝硬化模型组(CIRM组)、肝硬化模型+左旋硝基精氨酸甲酯(L-NAME)+锌原卟啉-Ⅸ(Znpp-Ⅸ)组(CIRM+L-NAME+ZnPP-Ⅸ组)。采用复合因素法建立大鼠肝硬化模型。运用敏感硫电极方法检测大鼠血浆中H2S的含量;应用免疫组织化学方法对大鼠门静脉(PV)上CSE蛋白进行定位及半定量分析;利用蛋白质免疫印迹技术(Western blot)测定大鼠肝组织中CSE蛋白的表达量。结果:在大鼠肝硬化形成后,血浆H2S含量显著降低,CIRM组〔(134.49±12.25)umol/L〕与NC组〔(180.34±11.71)umol/L〕比较有显著性差异(P<0.01);门静脉上CSE蛋白的表达〔NC组、CIRM组分别为(139.19±1.13)、(153.68±0.90)〕及其在肝组织中的蛋白含量〔NC组、CIRM组分别为(159.30±1.37)、(121.72±1.61)〕显著降低(P<0.01);当外源性给予L-NAME、ZnPP-Ⅸ后,CIRM+L-NAME+ZnPP-Ⅸ组血浆H2S含量〔(160.81±6.79)u-mol/L〕显著高于CIRM组〔(134.49±12.25)umol/L,P<0.01〕,同时,CIRM+L-NAME+ZnPP-Ⅸ组门静脉上CSE蛋白的表达(148.72±1.39)及其在肝组织中的蛋白含量(142.79±1.13)显著高于CIRM组(P<0.01)。结论:在大鼠肝硬化形成中,NO、CO可以上调H2S/CSE体系。Objective：To explore the effect of nitric oxide（NO） and carbon monoxide（CO） on the hydrogen sulfide（H2S）/ cystathionine-γ-lyase（CSE） system in hepatic cirrhosis of rats.Methods：Sprague-Dawley（SD） rats were randomly divided into three groups：normal control group（NC）,cirrhosis model group（CIRM）,cirrhosis model＋ L-NAME＋ ZnPP-Ⅸ group（CIRM＋ L-NAME＋ ZnPP-Ⅸ）.The hepatic cirrhosis model of rats were established by complex pathogens.The content of H2S in plasma of rats was measured by sulfide electrode method.The expressions and location of CSE in the portal vein（PV） were studied by immunohistochemistry.Western-blot was used to analyze hepatic tissue CSE protein expression.Results：The plasma H2S 〔（134.49±12.25）umol/L〕of CIRM group was decreased significantly compared with NC group〔（180.34±11.71）umol/L,P0.01〕.The expression of CSE in PV and hepatic tissue CSE protein expression in CIRM group〔（153.68±0.90）（,121.72±1.61）,respectively〕were significantly decreased compared to NC group〔（139.19±1.13）,（159.30±1.37）,respectively：all P0.01〕.Compared with CIRM group,the plasma concentration H2S was increased significantly in CIRM＋L-NAME＋ ZnPP-Ⅸ group〔（160.81±6.79）umol/L,P0.01〕.Meanwhile,The expression of CSE in PV and hepatic tissue CSE protein expression were significantly increased in CIRM＋L-NAME＋ ZnPP-Ⅸ group 〔（148.72±1.39）,（142.79±1.13）,respectively：all P0.01〕.Conclusion：NO、CO upregulated H2S/CSE system in hepatic cirrhosis of rats.

关 键 词：一氧化氮 一氧化碳 硫化氢 胱硫酶-γ-裂解酶 肝硬化 

分 类 号：Q95-3[生物学—动物学] R575.2[医药卫生—消化系统]