至真方对人大肠癌细胞株HCT8/VCR多药耐药的逆转作用及P-gp表达的影响  

Reversal of Multidrug Resistance and Expression of P-gp on Human Colorectal Cancer Cell line HCT-8/VCR by Zhizhen Recipe

作　　者：陈志霞[1] 孔令春[1] 孙玉舫[1] 王松坡[1] 

机构地区：[1]上海交通大学附属第一人民医院中医科,上海200080

出　　处：《现代生物医学进展》2010年第7期1242-1246,共5页Progress in Modern Biomedicine

摘　　要：目的:研究至真方对大肠癌耐药细胞株HCT-8/VCR的逆转作用及P糖蛋白(P-gp)表达的影响。方法:MTT法测定至真方含药血清对HCT-8/VCR细胞株的逆转作用,并以流式细胞术测定P-gp的表达。结果:经至真方含药血清作用72h后,HCT-8/VCR细胞株对化疗的耐药性显著下降,对长春新碱逆转指数为0.937±0.093,其逆转倍数大于13.6倍,与维拉帕米比较无统计学差异(P>0.05)。HCT-8/VCR细胞的P-gp表达显著高于HCT-8(P<0.01),经至真方含药血清作用后P-gp表达显著降低(P<0.01)。结论:至真方可以逆转人大肠癌耐药细胞株HCT-8/VCR的多药耐药性,其机制可能与其下调P-gp的表达有关。Objective：To investigate the reversal effect of Zhizhen Recipe on multidrug resistance of human colorectal cancer cell line HCT-8/VCR and the impact of Zhizhen Recipe to the expressions of P-gp inHCT-8/VCR.Methods：The reversal effect of Zhizhen Recipe was observed by MTT assay,and the expressions of P-gp in HCT -8/VCR cell line was detected with flow cytometry（FCM）.Re sults：After stimultaneous treatment for 72 hours with Zhizhen Recipe,the sensitivity of HCT-8/VCR rose significantly to VCR.The reversal index（RI）to VCR was 0.937±0.093,and there are no statistical significance compared VER group（P0.05）.The Pgp had higher expression in HCT-8/VCR than in HCT-8（P0.01）.It showed significant dicrease after Zhizhen Recipe treatment（P0.01）.Conclusion：Zhizhen Recipe can reverse the resistance of HCT-8/VCR cell line.The mechanism may be related to the effect of down-regulation P-gp.

关 键 词：至真方 药耐药 大肠癌 P糖蛋白 

分 类 号：R735.34[医药卫生—肿瘤] R285.5[医药卫生—临床医学]