ras蛋白在食管癌及食管癌前病变组织中的表达  

Expression of ras protein in human esophageal cancerous and precancerous lesions

作　　者：李健[1,2] 赵治国[1,2] 周琦[1,2] 王立东[1,2] 

机构地区：[1]河南医科大学第二附属医院消化内科 [2]河南医科大学癌症研究室

出　　处：《中国肿瘤临床与康复》1999年第1期11-12,共2页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：国家自然科学基金

摘　　要：目的了解中国食管癌高发区食管癌变发生过程中癌基因ｒａｓ的变化。方法采用卵白素－生物素－辣根过氧化氢酶复合物（ＡＢＣ）方法测定食管癌高发区食管癌及癌前病变组织中癌基因ｒａｓ蛋白的表达状况。结果在正常食管上皮和癌前病变组织中未见ｒａｓ蛋白表达，而３６例食管癌组织有９例出现免疫阳性反应，其免疫阳性反应率为２５％。结论ｒａｓ癌基因表达是食管癌变晚期阶段的一个分子学变化。Objectives To understand the alterations of oncogene ras in esophageal carcinogenesis in a high incidence area of esophageal cancers in northern of China.Methods The expression of oncogene ras was determined in esophageal cancer Qus and precancerous lesions collected from the subjects in a high incidence area of esophageal cancers with avidin biotin peroxidase complex(ABC) method.Results In the normal tissues and the tissues with different severity of lesions from esophageal biopsies,the expression of ras protein was not detected.ras protein immunoreactivity was observed in 9 of the 36 surgically resected esophageal cancer specimens,accounting for 25%.Conclusions The present studies indicated that the expression of oncogene ras was one of the molecular changes in the late stage of esophageal carcinogenesis.

关 键 词：食管癌 癌基因 RAS蛋白 癌前病变 

分 类 号：R735.102[医药卫生—肿瘤]