小鼠软骨发育的形态学观察  

Morphological Observation of Cartilage Development in Mice

作　　者：张桂林[1] 谭炳海[1] 商好敏[2] 曹荣峰[1] 

机构地区：[1]青岛农业大学动物科技学院,青岛266109 [2]南京农业大学动物科技学院,南京210095

出　　处：《中国畜牧兽医》2010年第5期54-56,共3页China Animal Husbandry & Veterinary Medicine

摘　　要：应用茜素红—阿利新蓝双重染色技术制作骨与软骨透明标本,观察幼龄小鼠软骨发育的形态学变化。取1、3、5、7、9、15日龄完整幼鼠,水淹致死后去除皮肤、眼、脑、内脏和脂肪组织,乙醇固定,丙酮脱脂,自来水冲洗,茜素红—阿利新蓝混合染色,氢氧化钾透明,各梯度甘油脱水,纯甘油保存。结果小鼠骨骼双重染色硬骨显示为红色,软骨显示为蓝色。随着小鼠日龄的增长,小鼠肋软骨的骨化最为明显,后肢长骨末端的骺软骨板和髋部软骨骨化过程清晰可见。此试验可以清晰的显示不同日龄小鼠肋软骨、后肢关节软骨、髋部软骨的骨化过程。The transparent specimens of skeleton and cartilage were made by double-staining technique with alizarin red and alcian blue to observe the morphological changes of cartilage development in young mice. The whole mice taken at the days of 1,3,5,7,9,15 were flushed to death and the skin,eyes,brain,internal organs and adipose tissue were removed. The bone was stained into red and cartilage into blue. The ossific process of costal cartilage was the most obvious as the mice grew; the ossification of epiphyseal cartilage plate at the end of long bone in the posterior limb and the cartilage in the hip were clearly to be observed. The ossific process of costal cartilage,articular cartilage in the posterior limb and the cartilage in the hip were revealed clearly.

关 键 词：小鼠 软骨 发育 形态学 

分 类 号：S865.13[农业科学—野生动物驯养]