高效液相色谱法同时测定维生素B_6中两种生物活性成分  

Determination of Two Active Ingredients in Vitamin B_6 Formulations by High Performance Liquid Chromatography

作　　者：郭巧珍[1] 魏萍[2] 关勇彪[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]总后勤部卫生部药品仪器检验所,北京100071

出　　处：《解放军药学学报》2010年第1期68-69,72,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立同时测定血浆中两种不同形式维生素B6（吡哆醇Pyridoxine,PN和吡哆醛Pyridoxal,PL）的高效液相色谱法。方法采用Luna C18色谱柱（150 mm×4.6 mm,5μm）,外标法,荧光检测器（波长为λE326 nm,λM400 nm）,流动相：甲醇-磷酸缓冲液（12∶88）,pH3.2,流速：1.2 ml/min进行分析。结果在2.5~160μg/ml浓度范围内,血中PN、PL测定的平均回收率分别为（86.0±4.74）%和（96.1±3.52）%,RSD分别为4.25%和3.85%。血中PN、PL的最低检出浓度分别是2.37、1.28μg/ml。结论该法灵敏、快速、准确、重复性好。Objective To establish a High Pressure Liquid Chromatography method by which different types of Vitamin B6（such as Pyridoxine,PN and Pyridoxal,PL） in blood could be determined simultaneously.Methods in the outside-standardizing method,samples were determined using Luna C18 analysis column（150 mm×4.6 mm,5 μm） and fluorescence detector（λE 326 nm;λM 400 nm） with a mobile phase consisting of 35mmol/L sodium phosphate containing 2.5mmol/L heptanesulfonic acid,adjusted to pH 3.2 with 85% orthophosphoric acid and 12% methanol（V∶V） at 1.2ml/min.Results In the concentration range of 2.5 to 160 μg/ml,the average recycle rates of PN and PL in blood were（84.62±3.603）% and（95.66±3.695）%.The variation index of repeated determinations was 4.25% and 3.85% respectively.The detectable limit inferior concentration of PN and PL in blood was 2.37 and 1.28 μg/ml respectively.Conclusion A determination method of PN and PL by HPLC was constructed,being sensitive,accurate,quick and reproducible.

关 键 词：维生素B6 大鼠血浆 高效液相色谱法 吡哆醇 吡哆醛 

分 类 号：R927.2[医药卫生—药学]