五种重金属离子对克氏原螯虾(Procambarus clarkiai)的急性毒性作用研究  

Studies on Acute Toxicity of Five Heavy Metal Ion on Porcambarus clarkia Girard

作　　者：宋维彦[1] 靳桂双 毕伟伟[2] 田艳苓[2] 王慧[2] 

机构地区：[1]日照职业技术学院水产学院,山东日照276826 [2]山东农业大学动物科技学院,山东泰安271018

出　　处：《激光生物学报》2010年第2期206-211,共6页Acta Laser Biology Sinica

基　　金：山东省高等学校优秀青年教师国内访问学者项目;山东省博士后创新基金项目(200703044)

摘　　要：采用静水生物法、充气和恒温法研究了5种重金属离子对克氏原螯虾的急性毒性作用,并以直线内插法得出了24 h、48 h、72 h和96 h的半致死量(LD50)。研究结果表明:重金属离子的毒性顺序由大到小依次为:Hg^(2+)、Cd^(2+)、Cu^(2+)、Pb^(2+)和Zn^(2+)。根据毒性分级标准,5种重金属离子对克氏原螯虾均为高毒物,其安全质量浓度依次为:0.0143 mg/L、0.0322 mg/L、0.0401 mg/L、0.1995 mg/L和0.2795 mg/L。以浓度对数和死亡率进行回归分析,分别得出了5种重金属离子对螫虾24 h、48 h和96 h的回归方程。The acute toxicity of Hg2 ＋ , Cd2＋ , Cu2 ＋ , Pb2 ＋ and Zn2 ＋ were examined for Porcambarus clarkia Girard in terms of different consistency, through liner interpolation the LD50 of the five heavy metal ions were obtained to the crawfish by stagnophile test, gas filling and constant temperature methods. Results showed that the order of the toxicities of these heavy metals was Hg2＋, Cd2＋ , Cu2 ＋ , Pb2＋ and Zn2＋ in turn. According to the chemical substances toxic grading standards, the five heavy metal ions to the crawfish on the toxicity were all highly toxic, and the safety concentrations （SC） of Hg2＋ , Cd2＋ , Pb2＋ , Cu2＋ and Zn2＋ to the crayfish were 0. 0143 mg/L, 0. 0322 mg/L, 0. 0401 mg/L, 0. 1995mg/L, and 0. 2795 mg/L, respectively. Then based on the concentration logarithm and mortality regression a- nalysis, the 24 h, 48 h, and 96 h ot＇ the regression equation and correlation coefficient for Porcambarus elarkia Girard were obtained respectively.

关 键 词：重金属离子 克氏原螯虾 急性毒性实验 半致死量 安全质量浓度 

分 类 号：Q958.12[生物学—动物学]