贫营养异养硝化细菌的分离鉴定及硝化性能研究  

Nitrifying characteristic of oligotrophic heterotrophic nitrification bacteria

作　　者：苏俊峰[1] 黃廷林 李倩[1] 叶羡婧[1] 魏全源[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055

出　　处：《环境污染与防治》2010年第5期4-6,13,共4页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.50778147);教育部博士点新教师基金资助项目(No.20096120120013);陕西省教育厅专项科研计划项目(No.09JK540);西安建筑科技大学人才基金资助项目(No.RC0727)

摘　　要：从水库底泥中分离出1株贫营养异养硝化细菌,命名为菌株Y11。经菌株生理特性研究和16SrDNA测序,鉴定出菌株Y11属于假单胞菌属细菌(Pseudomonas sp.)。考察了温度、pH及C/N对菌株Y11的硝化性能影响,结果表明,菌株Y11进行硝化作用的最适温度为30℃,最适pH为中性,最适C/N为6.0,在该条件下进行4d的硝化反应,菌株Y11对氨氮去除率可达91.42%。The oligotrophic heterotrophie nitrifying bacterial strain Y11 was isolated from reservoir sediment. The strain Y11 was identified as Pseudomonas sp. according to its physiological and bioehemical index analysis and the 16S rDNA sequence analysis. The effects of temperature, pH, and C/N ratio on nitrifying characteristic of strain Y11 were investigated, and results showed that the optimal conditions for nitrification by strain Y11 were 30 ℃ ,neutral pH and C/N ratio of 6.0, under these conditions,the pure culture bacteria could degrade 91.42 % of ammonia-nitrogen in 4 d of nitrification process.

关 键 词：贫营养细菌 异养硝化 硝化特性 

分 类 号：X172[环境科学与工程—环境科学] X52