几种双孢蘑菇菌株对稻草降解能力的研究  

Studies on the Property of Some Agaricus spp.Strains in Degradation Rice Straw

作　　者：李燕荣[1,2] 周国英[1] 胡清秀[1] 赵亮亮[3] 

机构地区：[1]中南林业科技大学生命科学学院,湖南长沙410004 [2]中国农业科学院农业资源与农业区划研究所,北京100081 [3]广西师范大学环境与资源学院,广西桂林541004

出　　处：《北方园艺》2010年第10期207-210,共4页Northern Horticulture

基　　金："十一五"国家科技支撑计划资助项目(2007BAD89B1)

摘　　要：以蘑菇属的5个菌株为试材,采用Bavendamm显色反应、RB亮蓝平板变色反应、Eriksson变色圈试验及木质降解试验等方法,研究双孢蘑菇对稻草的降解能力。结果表明:通过Bavendamm平板和RB亮蓝平板显色试验,初步判断2796及A5,优于其它菌种,有较高的木质素降解相关酶系(木质素降解酶;木质素过氧化物酶;锰依赖过氧化物酶)活力;这2种菌种进一步通过变色圈和降解试验,表明2796为选择性降解纤维素的菌种,而A5为选择性降解木质素的菌种,但2796对木质素的降解率为39.82%,A5仅为20.91%,说明菌种对木质素的选择性降解与否与其降解率之间没有相关性。To promote the yield of Agaricus bisporus and improve efficiently using of farmland straw, 5 Agaricus bisporus strains（2796, A5, A2, A3 and Ar）was investigated about the function of degradation rice straw. In this experiment, Bavendamm chromogenic reaction, RB bright blue flat color reaction, Eriksson discoloration circle test and Woodiness degradation test was finished. The results showed that A5 and 2796 had higher relevant lignin degrading enzymes including lignin degradation enzyme, lignin peroxidase, manganese dependence peroxidase vitality through the Bavendamm chromogenie reaction and RB bright blue flat color test;then all the strains by Eriksson discoloration circle test and Woodiness degradation test appeared that 2796 and A3 strains were selective degradation of cellulose in the beginning of the experiment, however the others was selective degradation of lignin, and the highest degradation rate of lignin of 2796 was 39.82%, A5 was 20. 91% just only, those results explained to us that there was no correlation between the selectivity of lignin and degradation rate of it. Finally we can selected that 2796 and A5 were the most efficient degradation of rice straw stains.

关 键 词：双孢蘑菇 Bavendamm显色 RB亮蓝平板变色 Eriksson 变色圈试验 木质降解 

分 类 号：S646.11[农业科学—蔬菜学]