重庆地区人群HLA-A,B基因和单倍型的多态性分析  

Research of HLA-A genes,B genes and analysis on haplotype′s polymorphism of Chongqing

作　　者：陈伟[1] 邓少丽[1] 袁琳[1] 

机构地区：[1]第三军医大学大坪医院检验科,重庆400042

出　　处：《重庆医学》2010年第10期1182-1183,共2页Chongqing medicine

基　　金：第三军医大学科研基金资助项目(2008-1087)

摘　　要：目的对重庆地区人群HLA-A,B基因座位的单倍型分布特征及基因相关性进行研究。方法采集重庆地区201名无血缘关系健康个体静脉血,应用聚合酶链反应-序列特异性引物(PCR-SSP)方法对其HLA-A,B基因座位进行等位基因分型,计算HLA-A,B基因型频率及基因频率,统计HLA-A,B单倍型出现情况并做连锁相关性分析。结果在重庆地区的人群中,最常见的HLA-A-B单倍型是HLA-A*02-B*46、HLA-A*11-B*13和HLA-A*24-B*46、HLA-A*30-B*13。HLA-A*02-B*46、HLA-A*11-B*60和HLA-A*30-B*13为重庆分库最常见、连锁不平衡最显著的单倍型。与我国北方、南方汉族比较,更接近南方汉族人群。结论重庆地区人群HLA-A,B基因具有较为丰富的多态性,其分布符合南方汉族的特点,HLA单倍型分布特征与南方汉族人群相近,但也有其自身特点。Objective To investigate the gene locuses haplotype distributing character and to analyse the gene′s relativity of HLA-A genes,B genes in Chongqing.Methods A PCR-based sequence specific primersmethod was used to determine the alleles of HLA-A,B in 201 unrelated,healthy people of Chongqing.Allelic frequencies were calculated by the direct count and stated HLA-A,B haplo-type and analyzed the concatenation relativity.Results The people of Chongqing,the most frequent HLA-A-B haplotypes were HLA-A＊02-B＊46,HLA-A＊11-B＊13,HLA-A＊24-B＊46,HLA-A＊30-B＊13,and HLA-A＊02-B＊46,HLA-A＊11-B＊60 and HLA-A＊30-B＊13 were the most frequent in Chongqing area and had significant linkage disequilibrium.Conclusion HLA-A,B in people of Chongqing have plenty of polymorphism and distribute in line with the southern Han′s characteristics.

关 键 词：HLA抗原 单倍型频率 遗传多态性 

分 类 号：R394[医药卫生—医学遗传学]