利用籼粳稻特异InDel标记分析云南糯稻品种的籼粳特性  

Characteristic of Yunnan Glutinous Rice Varieties Revealed by the Indica-Japonica Specified Insertion/Deletion (InDel) Molecular Markers

作　　者：王明军[1] 王云月[1] 陆春明[1] 杨慧[1] 王云涛[1] 

机构地区：[1]云南农业大学植物保护学院,农业生物多样性应用技术国家工程研究中心,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2010年第3期333-337,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家“973”项目(2006CB100205)

摘　　要：利用籼粳杂种优势是实现水稻超高产育种目标的重要途径之一。利用根据粳稻日本晴和籼稻93-11基因组序列设计的34对特异性插入/缺失(Insertion/Deletion,InDel)引物,对采自云南省7个地州17个县的181份糯稻品种进行籼粳性分析。通过对这些糯稻品种DNA样品在这34个InDel位点上的籼型或粳型基因频率,确定各个糯稻品种的籼、粳性。结果表明:141份测试品种被鉴定为典型籼稻,18份为籼稻,8份为典型粳稻,13份为粳稻,1份为偏粳稻。主成分分析表明,云南糯稻籼粳分化明显。Utilization of Indica-Japonica heterosis was one of the most important ways to achieve the goal of high yield rice breeding.Indica-Japonica classifications of 181 glutinous rice varieties collected in 17 counties of 7 cities in Yunnan were analyzed by calculating Indica and Japonica gene frequencies on 34 InDel sites of each glutinous rice varieties DNA samples and principal component analysis（PCA）.181 Yunnan glutinous rice varieties were classified as 141 typical Indica rice,18 Indica rice,1 Japonicaclinous rice,13 Japonica rice and 8 typical Japonica rice.PCA showed that differentiations of Yunnan glutinous rice were significant.

关 键 词：籼粳分化 INDEL标记 糯稻 

分 类 号：S511.032[农业科学—作物学]