口蹄疫病毒非结构蛋白3AB、3A基因的克隆与原核表达  

Clone and Prokaryotic Expression of Non-Structure Protein 3AB & 3A Genes in Foot-and-Mouth Disease

作　　者：张先锋[1,2,3] 易忠[1,3] 魏玉荣[1,3] 胡尔玛西[1,3] 符子华[1,3] 

机构地区：[1]新疆畜牧科学院兽医研究所,乌鲁木齐830000 [2]新疆农业大学动物医学学院,乌鲁木齐830000 [3]新疆动物生物技术重点开放实验室,乌鲁木齐830000

出　　处：《新疆农业大学学报》2010年第3期210-213,共4页Journal of Xinjiang Agricultural University

基　　金：新疆维吾尔自治区高技术研究发展项目(200911111)

摘　　要：以构建的口蹄疫病毒非结构蛋白3ABC质粒pGEM-T-3ABC为模板,设计了特异性的引物,进行RT-PCR扩增得到非结构蛋白3AB、3A基因,然后分别定向克隆到原核表达载体pET-28a、pET-41a载体上,将重组质粒转化宿主菌BL21,IPTG诱导表达目的蛋白。表达产物经SDS-PAGE和Western blotting检测,证明重组蛋白在大肠杆菌中成功表达。Based on the construction of template of non-structure protein 3ABC and plasmid pGEM-T- 3ABC,the exceptional primers were designed and amplified by RT-PCR to get the non-structure protein 3AB and 3A genes. They were directed to be cloned into prokaryotic expression carrier pET-28a and pET-41a respectively. The recombinant plasmids were transformed into hose BL21, and the target proteins were induced by IPTG in BL21. The expressed proteins were examined by SDS-PAGE and Western blotting. The result showed that recombinant proteins were expressed in E. coli successfully.

关 键 词：口蹄疫病毒 非结构蛋白 克隆 表达 

分 类 号：S851.659.6[农业科学—预防兽医学]