不同剂量海人酸海马注射建立大鼠颞叶癫痫模型的实验研究  

An experimental study on different doses of kainic acid inducing epilepsy in rats

作　　者：张玉奇[1] 刘卫平[1] 王彦刚[1] 郑朝辉[1] 刘阳[1] 龙乾发[1] 

机构地区：[1]第四军医大学西京医院神经外科,西安710032

出　　处：《山西医科大学学报》2010年第5期383-386,共4页Journal of Shanxi Medical University

基　　金：陕西省科学技术发展计划资助项目(2008K09-09)

摘　　要：目的通过比较建模成功率,找到建立大鼠海人酸(kainic acid,KA)颞叶癫痫模型的合适用药剂量。方法雄性SD大鼠80只,体重240-260g,随机分为4组,每组20只,分别向右侧海马注射KA1.0μl,0.7μl,0.4μl和生理盐水1.0μl,KA浓度0.5μg/μl,术后观察4h,按照Racine的癫痫大鼠发作时的行为学分级标准,连续3次达到Ⅳ-Ⅴ级发作者确定成功模型。结果观察结束时,生理盐水组大鼠全部存活且未见痫性发作。KA1.0μl组死亡14只,Ⅴ级发作6只。KA0.7μl组死亡2只,Ⅴ级发作15只,Ⅳ级发作3只。KA0.4μl组死亡1只,Ⅴ级发作1只,Ⅳ级发作6只,Ⅲ级发作9只,Ⅱ级发作3只。经统计学检验,KA0.7μl组大鼠颞叶癫痫模型的成功率最高。结论 0.7μlKA右侧海马注射较易获得大鼠颞叶癫痫模型。Objective To explore a proper dose of kainic acid （KA） inducing epilepsy model in rats. Methods Eighty male SD rats,weighing 240-260 g,were randomly divided into 4 groups（n=20 in each group）.Then the kainic acid at the concentration of 0.5 μg/μl for 1.0 μl,0.7 μl,0.4 μl and saline 1.0 μl were respectively injected into the right hippocampus of rats in 4 groups.After the injection,the behaviors of all rats were recorded for 4 h according to Racine＇s classification standard （1972）.The rat with 3 consecutive seizures of Grade Ⅳ-Ⅴ was defined as a successful model of epilepsy. Results In saline group,all rats survived,and no seizure was observed.In KA 1.0 μl group,14 rats died,and 6 rats reached grade Ⅴ.In KA 0.7 μl group,2 rats died,15 rats reached grade Ⅴ,and 3 rats reached grade Ⅳ.In KA 0.4 μl group,1 rat died,1 rat reached grade Ⅴ,6 rats reached grade Ⅳ,9 rats reached grade Ⅲ and 3 rats reached grade Ⅱ before the end of the observation. Conclusion KA 0.7 μl has the best effect for establishing the model of epilepsy.

关 键 词：癫痫 大鼠 海人酸 

分 类 号：R742.1[医药卫生—神经病学与精神病学]