两种饮食限制方式诱导大鼠肝脏细胞自噬的分析  

Comparison of autophagy induced by two approaches of dietary restriction in hepatocytes of rats

作　　者：王慧俐[1] 邱龄[1] 李艳芳[1] 

机构地区：[1]山西医科大学第二临床医学院心内科,太原030001

出　　处：《山西医科大学学报》2010年第5期396-399,共4页Journal of Shanxi Medical University

基　　金：山西省基础研究计划项目(自然)(2010011054-2)

摘　　要：目的观察两种饮食限制方式下各组大鼠肝脏细胞自噬的程度,以期探索饮食限制与细胞自噬的关系。方法 24月老龄大鼠按体重随机分为A组(正常饮食对照组)、B组(1d自由饮食1d禁食)、C组(5d自由饮食2d禁食),每组10只。饲养5个月后活体状态下取1mm3肝脏组织4℃保存,50mg肝组织-70℃冻存。透射电镜观察各组肝脏细胞自噬泡的数量;RT-PCR检测微管相关蛋1-轻链3(MAP1-LC3)的mRNA表达。结果与A组相比,B组和C组自噬泡占细胞质总面积的比值均明显提高(P<0.01),而两组间该比值差异无统计学意义(P>0.05)。B组和C组MAP1-LC3的表达较A组显著升高(P<0.01),而两组间差异无统计学意义(P>0.05)。结论两种饮食限制方式使大鼠的肝脏细胞自噬程度明显增强,通过饮食限制可增强大鼠肝脏细胞的自噬程度。Objective To explore the relationship between autophagy of rat hepatocytes and dietary restriction. Methods Thirty 24-month-old rats were randomly devided into three groups：group A （normal diet）,group B （1 d free diet and 1d fasting）,group C （5 d free diet and 2 d fasting）.After feeding for five months,in vivo 1 mm3 liver tissues were taken and stored at 4 ℃,while 50 mg liver tissues were stored at -70 ℃.The amount of autophagosomes of hepatic cells was detected by transmission electron microscope（TEM）.RT-PCR was used to detect the expression of microtubule-associated protein 1-light chain 3（MAP1-LC3）mRNA. Results Compared with group A,the ratio of autophagosomes to that of total cytoplasm increased significantly in both group B and group C（P〈0.01）,but there was no significant difference between group B and group C（P〈0.05）.MAP1-LC3 expression was significantly higher in both group B and group C than in group A（P〈0.01）,while no statistical significance between group B and group C（P〈0.05）. Conclusion The degree of autophagy of hepatocytes obviously increases in rats with dietary restrictions.The results suggest that dietary restrictions can improve the autophagy of rat hepatocytes.

关 键 词：细胞自噬 饮食限制 透射电镜 微管相关蛋1-轻链3 

分 类 号：R363[医药卫生—病理学]