移植的嗅鞘细胞在大鼠正常和挫伤脊髓内的迁移  

Migration of Olfactory Ensheathing Cells Transplanted in Normal or Contused Spinal Cord of Rats

作　　者：张立仁[1] 叶超群[1] 孙天胜[1] 包建玲[2] 刘彦[2] 

机构地区：[1]北京军区总医院骨科,北京市100700 [2]北京市虹天济神经科学研究院,北京市100045

出　　处：《中国康复理论与实践》2010年第5期429-431,F0003,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金项目(30872604);北京市委组织部优秀人才培养计划项目(20071D0502000266);北京市虹天济神经科学研究院科研基金资助项目

摘　　要：目的明确移植的嗅鞘细胞在脊髓内的迁移特点。方法来源于7日龄绿荧光Sprague Dawley大鼠嗅鞘细胞分别移植于正常大鼠脊髓、脊髓挫伤模型大鼠脊髓损伤部位中心及其临近组织,1周后取材观察移植细胞在脊髓内的迁移能力。结果移植嗅鞘细胞在正常脊髓内主要沿脊髓纵轴方向从注射位点向两侧在白质和灰质内迁移,绝大部分迁移细胞排列无规则;移植于脊髓损伤部位中心的嗅鞘细胞主要围绕注射位点沿脊髓纵轴呈椭圆形分布,占据损伤部位并分布于其周围的脊髓内,小部分在白质内向头侧和尾侧迁移,迁移距离较短;移植于距离损伤部位头侧和尾侧1mm处的嗅鞘细胞的主要在白质和灰质内沿脊髓纵轴向损伤部位迁移,还分别有一小部分沿中央管迁移。移植细胞可表达髓磷脂相关蛋白和神经丝。结论移植的嗅鞘细胞可在脊髓内迁移,其迁移能力取决于其自身特性,移植环境是影响其迁移的重要因素。Objective To explore the migration,effect on axon growth of olfactory ensheathing cells （OECs） transplanted in contused spinal cord of rats.Methods 8 adult female rats were induced spinal cord contusion at T10 cord by NYU impactor （H=25 mm）,and received OECs transplantation in 1 mm rostral and caudal to injury site,or injury site.Other 4 adult female rats were uncovered the spinal cord at T8-10 cord,and injected GFP＋OECs at T10 cord.1 week after transplantation,all animals were executed and the T8-11 cord （15 mm long） contained the entire injury site were observed for the migration of OECs and immunostained for neurofilament （NF） and myelin basic protein （MBP）.Results The OECs injected in injury site largely migrate longitudinally and laterally from the injection site,OECs injected in 1 mm rostral and caudal to injury site of contused spinal cord,migrate longitudinally and laterally from the injection site to the injury site in white and gray matter,and some along the central canal.OECs injected in normal spinal cord migrated longitudinally and laterally from injection site,too.The transplanted OECs expressed a little NF and MBP.Conclusion The transplanted OECs are able to migrate in spinal cord and promote axon regeneration and remyelination.

关 键 词：脊髓挫伤 嗅鞘细胞 移植 迁移 

分 类 号：R734.2[医药卫生—肿瘤]