慢性吗啡成瘾小鼠海马中RACK1对CREB表达水平的影响  

Effects of protein receptor for activated C kinase 1 (RACK1) on CREB expression in hippocampus of mice with chronic morphine dependence

作　　者：谢一舟[1] 万莉红[1,2] 苏岚[1] 汪宇辉[1] 刘延友[1] 朱雨[1] 王正荣[1] 

机构地区：[1]四川大学时间生物学卫生部重点实验室 [2]四川大学华西基础与法医学院药理教研室,四川成都610041

出　　处：《四川生理科学杂志》2010年第2期51-54,共4页Sichuan Journal of Physiological Sciences

基　　金：国家自然科学基金资助(编号:30470684)

摘　　要：目的:研究shRAKC1对慢性成瘾小鼠脑组织CREBmRNA、蛋白的表达变化。方法:通过条件性位置偏爱(CPP)实验分析shRAKC1对慢性吗啡成瘾小鼠的作用;通过RT-PCR检测RACK1和CREB的mRNA在成瘾小鼠海马中的表达水平,并采用免疫组化观察RACK1和CREB蛋白的表达情况。结果:慢性成瘾组与生盐水组相比,前者海马区RACK1和CREBmRNA表达升高(p<0.05),且吗啡诱导的CPP效应增强(p<0.05),而shRAKC1组与空质粒组相比,前者由吗啡诱导的CPP诱导效应减弱(p<0.05),同时其海马区RACK1和CREBmRNA表达下降(p<0.05)。结论:干扰慢性吗啡成瘾小鼠RACK1表达,可以使CREB表达水平下调,并有抑制吗啡成瘾效应的作用。Objective： To investigate the effects of shRACK1 on CREB in hippocampus of mice. Method： Analysing the effects of CPP in hippocampus of mice. The expression of RACKI and CREB were determined by RT-PCR and Immunohistochemistry. Result： The level of RACK1 and CREB mRNA in morphine-induced group is higher than in the saline group（p〈0. 05）, morphine-indueed group＇s CPP is increased（p〈0. 05）. And the level of RACK 1 and CREB mRNA in control plasmid group is higher than in shRACK1 group（p〈0. 05）, morphine-induced group＇s CPP is decreased（p〈0. 05）. Conclusion： shRACK1 in mice with chronic morphine dependence can decrease the expression of CREB, and it can decrease the effects of chronic morphine dependence.

关 键 词：慢性吗啡成瘾 海马RACK1 CREB 

分 类 号：R749.6[医药卫生—神经病学与精神病学]