急性血栓性肺栓塞兔体内肝细胞生长因子的变化  

Change of hepatocyte growth factor levels in rabbit with acute pulmonary thromboembolism

作　　者：梁明亭[1] 李义波[1] 杨永[1] 侯新华[1] 孔静博[1] 徐俊龙[2] 

机构地区：[1]聊城市人民医院心内科,山东聊城市252000 [2]聊城市人民医院病理科,山东聊城市252000

出　　处：《中华老年多器官疾病杂志》2010年第1期73-76,78,共5页Chinese Journal of Multiple Organ Diseases in the Elderly

摘　　要：目的观察实验性急性血栓性肺栓塞(PTE)对兔血清和组织中肝细胞生长因子(HGF)水平的影响,探讨HGF对于肺栓塞的诊断价值。方法将32只家兔随机分为血清HGF检测组(16只)和组织HGF检测组(16只),每组又分为对照组和实验组各8只。实验组采用自体血栓回输法建立肺栓塞模型,对照组以与自体血栓等量生理盐水注入。ELISA法检测0,1,3,6,12,24,48,72h血清HGF含量;组织HGF检测组12h处死家兔,取其肺、右心和肝组织,免疫组织化学法检测其HGF的表达。结果模型组血清HGF水平显著高于对照组,并在家兔PTE后1h开始升高,血清HGF含量在6,12,24,48h升高幅度较大,于12h升高最明显,与对照组比较差异显著〔(1.7±0.5)vs(0.26±0.23)μg/L,P<0.001〕,72h开始回落。对照组则在较低水平(<0.5μg/L)。模型组肺和右心组织HGF的表达量明显高于对照组和其肝组织。两组间肝脏HGF的表达没有显著差异。HGF在肺组织的表达明显高于右心和肝组织。结论HGF可作为早期PTE的辅助诊断。Objective To study the influence of experimental acute pulmonary thromboembolism （PTE） on hepatocyte growth factor （HGF） levels in plasma and tissue of rabbit,and to explore the diagnostic value of HGF in PTE. Methods Thirty-two rabbits were randomly used for detection of HGF in plasma （n=16） and tissues （n=16）. For each detection,the rabbits were further divided into control group and PTE model group,with 8 in each. The PTE model was established by intravenous injection of autologous blood clots,and the control group was injected with isometric normal saline. The HGF （0,1,3,6,12,24,48,72 h） level in plasma was detected by ELISA. The rabbits were sacrificed at 12 h,and the expression of HGF in tissue of lung,liver,and right side of the heart was detected by immunohistochemistry. Results The plasma HGF level was significantly higher in PTE group than in control group. It began to elevate at 1h after PTE,and reached its peak at 12 h,significantly higher than that in control group〔（1.74±0.49） vs （0.26±0.23） μg/L,P0.001〕. Then,it began to reduce gradually at 72 h. However,it remained at low level in control group through the whole experimental duration （P0.5 μg/L）. In tissue of lung and right side of the heart,the expression of HGF was significantly higher in PTE group than in control group,while in liver tissue,it was not significantly different between PTE group and control group. The HGF expression in lung tissue was significantly elevated compared with right side of the heart and liver tissues. Conclusion HGF can be used for the adjunctive diagnosis of PTE.

关 键 词：肺栓塞 模型 动物 细胞因子类 肝细胞生长因子 

分 类 号：R363[医药卫生—病理学]