HPV16/18E6、cyclinD1、hTERT在喉癌组织中的表达及意义  

Correlation between expression of HPV16/18E6、cyclinD1、hTERT and development of laryngeal carcinoma

作　　者：吕彩凤[1] 江黎珠[1] 陈鸿雁[1] 

机构地区：[1]重庆医科大学附属第一医院耳鼻喉科,重庆400016

出　　处：《重庆医科大学学报》2010年第4期515-518,共4页Journal of Chongqing Medical University

摘　　要：目的:检测人乳头状瘤病毒(Human papillomavirus,HPV)16/18E(HPV16/18E6),周期素D1(CyclinD1)、端粒酶逆转录酶(Human telomerase transcriptase,hTERT)在喉癌组织中的表达水平,探讨HPV16/18E6、cyclinD1、hTERT与喉癌的关系。方法:收集手术标本的石蜡切片42例,用免疫组织化学法检测喉癌组织中HPV16/18E6、cyclinD1、hTERT的表达情况。结果:(1)在喉癌组织中,HPV16/18E6、cyclinD1、hTERT阳性表达率分别为42.9%(18/42)、50.0%(21/42)、83.3%(35/42),明显高于对照组。(2)在喉癌组织中,HPV16/18E6和cyclinD1阳性表达率随T分期的增加及淋巴结转移而升高。hTERT阳性表达率随T分期增加而升高,而与淋巴结转移无关。三者都与年龄及性别无关。(3)HPV16/18E与cyclinD1,HPV16/18E与hTERT两者相互间都呈正相关。结论:(1)HPV16/18E6、cyclinD1、hTERT的高表达可能与喉癌的发生及发展相关。(2)喉癌中HPV16/18E6与cyclinD1,HPV16/18E6与hTERT之间可能存在相互作用,这种相互作用可能导致喉癌的发生,这可能为HPV16/18与喉癌发生之间的关系研究提供重要信息。Objective：To evaluate the role of human papillomavirus（HPV）16/18E6,cyclin D1,and human telomerase transcriptase（hTERT）in the development of laryngeal carcinoma by detecting the expression of these three genes.Methods：Immunohistochemistry was used to detect the levels of HPV16/18E6,cyclin D1,and hTERT in 42 cases of paraffin-embedded tissues.Results：（1）The positive expression ratios of HPV16/18E6,cyclinD1,and hTERT in the laryngeal carcinoma group were 42.9%（18/42）,50%（21/42）,and 83.3%（35/42）,respectively.These values were significantly higher than that in the control group.（2）The increase in the expression of HPV16/18E6 and cyclinD1 was associated with T stage advance and lymph node metastases.The expression of hTERT increased along with T stage advance,but not with lymph nodes metastases.The changes in the expression of HPV16/18E6,cyclinD1,and hTERT were not associated with gender and age.（3）Positive correlation was observed between HPV16/18E6 and cyclinD1 as well as between HPV16/18E6 and hTERT in laryngeal carcinoma.Conclusion：（1）The expression of HPV16/18E6,cyclin D1,and hTERT may be associated with the development of laryngeal carcinoma.（2）There may be interactions between HPV16/18E6 and cyclinD1,between HPV16/18E6 and hTERT.These interactions may induce the development of laryngeal carcinoma.The information provided by this study may be important for further investigation into the relationship between HPV16/18 and laryngeal carcinoma.

关 键 词：喉癌 HPV16/18E6 CYCLIND1 HTERT 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤]