食管鳞癌组织中RHAMM和TPX2基因的表达及临床意义的研究  

Expression of RHAMM gene and TPX2 gene in esophageal squamous carcinoma and its clinical significance

作　　者：毛宁[1] 向小勇[1] 陈力[2] 沈学远[2] 洪斌[2] 

机构地区：[1]重庆医科大学附属第一医院心胸外科,重庆400016 [2]重庆市第二人民医院心胸外科,重庆400020

出　　处：《重庆医科大学学报》2010年第4期557-560,共4页Journal of Chongqing Medical University

摘　　要：目的:探讨透明质酸介导的细胞游走受体(Receptor for hyaluronan-mediated motility,RHAMM)和Xklp2靶蛋白(Targetingprotein for Xklp2,TPX2)基因在食管鳞癌中的表达及其与临床病理因素的关系。方法:采用逆转录聚合酶链反应技术,检测40例食管鳞癌肿瘤组织、癌旁组织、相应食管正常黏膜组织中RHAMM和TPX2基因的表达。结果:RHAMM mRNA在食管鳞癌组织中的表达(65.0%)显著高于癌旁组织(37.5%)及正常黏膜组织(22.5%),且与是否发生淋巴结转移有关(P<0.01);TPX2 mRNA在食管鳞癌组织中的表达(75.0%)也显著高于癌旁组织(47.5%)及正常黏膜组织(40.0%),与是否发生淋巴结转移及分化程度有关(P<0.05);RHAMM mRNA及TPX2 mRNA在食管鳞癌组织中的阳性表达具有相关性(P<0.01,r=0.39)。结论:RHAMM及TPX2基因可能共同参与了食管鳞癌的发生、进展及转移。Objective：Toinvestigate the expression of RHAMMgene and TPX2 gene in esophageal squamous cell carcinoma（ESCC）and their relationships with clinicopathological factors.Methods：The expression of RHAMM gene and TPX2 gene were detected by reverse transcriptase polymerase chain reaction（RT-PCR）in ESCC tissues,para-cancer tissues,and matched esophageal normal mucosa tissues of 40 patients.Results：The positive expressions of RHAMM mRNA in ESCC tissues（65.0%）were significantly higher than those in para-cancer tissues（37.5%）and esophageal normal mucosa tissues（22.5%）.Significant relationship was observed between positive expression of RHAMM mRNA and lymphnode metastasis（P〈0.01）.The positive expressions of TPX2 mRNA in ESCC tissues（75.0%） were also significantly higher than those in para-cancer tissues（47.5%）and esophageal normal mucosa tissues（40.0%）.Significant relationship was observed between positive expression of TPX2 mRNA and lymphnode metastasis or degrees of differentiation（P〈0.05）.A significant association was found between the mRNA expression of TPX2 and RHAMM（P〈0.01,r=0.39）.Conclusion：High expression of RHAMM gene together with TPX2 gene might play important roles in the tumorigenesis and metastasis of ESCC.

关 键 词：RHAMM基因 TPX2基因 食管鳞癌 RT-PCR 

分 类 号：R735.1[医药卫生—肿瘤]