5-羟色胺转运体基因第二内含子及启动子区多态性与自杀未遂的关联分析  

An analysis on the relationship between polymrhism of intron 2 in serotonin transporter and polymrhism of serotonin transporter gene and attempted suicide

作　　者：黎雪梅[1] 况利[1] 艾明[1] 

机构地区：[1]重庆医科大学附属第一医院心理卫生中心,重庆400016

出　　处：《重庆医科大学学报》2010年第4期568-571,共4页Journal of Chongqing Medical University

基　　金：重庆市卫生局科研项目(编号:07-2-049)

摘　　要：目的:探讨5-羟色胺转运体(Serotonin transporter,5-HTT)基因第二内含子多态性(Serotonin transporter gene 2 VNTR polymorphism,5-HTTVNTR)及启动子区多态性(Serotonin-transporter-linked promoter region,5-HTTLPR)与自杀未遂的相关性研究。方法:运用聚合酶链反应技术(Polymerase chain reaction,PCR)检测71例自杀未遂患者(患者组)和80名健康对照者(对照组)5-HTTVNTR及5-HTTLPR的3种基因型及等位基因分布频率。结果:自杀未遂组第二内含子基因型及等位基因分布频率与正常对照组之间无显著性差异;自杀未遂组与对照组5-HTTYLPR的基因型及等位基因(S/L)频率差异无统计学意义(χ2=1.08,P=0.58;χ2=0.99,P=0.32);进一步分析显示,有精神疾病自杀未遂组(37例)的短重复序列S等位基因频率为85.1%,与正常对照组S等位基因频率(72.5%)及无精神疾病自杀未遂组(69.1%)的差异均具有统计学意义(χ2=4.49,P=0.04;χ2=5.21,P=0.03)。结论:5-HTTLPR的S/S等位基因可能是自杀未遂的易感基因之一,而汉族人群中5-HTT基因第二内含子多态性与自杀未遂没有明显相关性。Objective：To explore the association between attempted suicide and polymrhism of intron 2 in serotonin transporter and polymrhism of serotonin transporter（5-HTTLPR）.Methods：Using the technique of polymerase chain reaction（PCR）,the variable number of tandem repeat polymorphism（5-HTTVNTR）and 5-HTTLPR was determined in 71 patients with attempted suicide subjects and 80 control subjects.The allelic and genetypes distributions in two groups were compared.Results：There were no significant differences in intron 2-VNTR genotype and allele frequency distributions between attempted suicide and healthy control subjects（χ^2= 1.08,P=0.58;χ^2=0.99,P=0.32）.The frequencies of allele S were significantly higher in suicides attempted suicide patients with psychiatric disorder（85.1%）than in normal controls（72.5%）and suicides without psychiatric disorde（r69.1%）（χ^2=4.49,P=0.04;χ^2=5.21,P=0.03）.Conclusion：The results suggested that the polymrhism of intron 2 of 5-HTT gene no associated with attempted suicide in the Han nationality.But the presence of 5-HTTLPR alleles S may increase the risk for attempted suicide.

关 键 词：自杀未遂 基因 多态现象(遗传学) 5-羟色胺转运体 

分 类 号：R195.1[医药卫生—卫生统计学]