牙龈卟啉单胞菌表面相关物质通过Fas-FasL途径诱导T淋巴细胞凋亡的体外研究  

The study of the apoptosis of T lymphocytes by the fas and fas ligand pathway induced by surface-associated material from porphyromonas gingivalis

作　　者：王惠宁[1] 潘乙怀[1] 赵瑜[1] 

机构地区：[1]浙江温州医学院附属口腔医院,325027

出　　处：《现代口腔医学杂志》2010年第3期198-201,共4页Journal of Modern Stomatology

基　　金：温州市科技计划项目(Y20080020)

摘　　要：目的检测Fas/FasL在牙龈卟啉菌表面相关物质(SAM)诱导的T淋巴细胞凋亡中的表达。方法选取10名全身及牙周组织健康受试者,分离外周血中单核细胞(PBMC),用SAM体外刺激PBMC,用特殊的单克隆抗体(Fas、FasL)标记,并进行流式细胞仪检测。结果 FasFasL的表达量明显上调。实验组Fas表达量在24h为25.38%±3.17,在96h为65.11%±3.58;实验组FasL的表达量在24h为5.23%±1.53,在96h为20.59%±2.68。用抗Fas单克隆抗体阻滞Fas-FasL相互作用导致T细胞凋亡的明显减少,百分比为20.53%±3.26,未加抗体的为48.47%±4.15。但残余的细胞凋亡活动与阴性对照相比仍高。结论 SAM诱导T淋巴细胞凋亡主要通过Fas-FasL途径,并具有时间依赖性,通过数据证明了细胞凋亡的分子机制。Objective To investigate the involvement of the Fas/FasL-mediated apoptotic pathway in surface-associated material from Porphyromonas gingivalis （SAM） -induced T-cell apoptosis.Methods Ten systemically and periodontally healthy subjects participated in this study. Venous blood was drawn and peripheral blood mononuclear cells （PBMC） was isolated. T lymphocytes stimulated with SAM and the cells were labled by the special monoclonal antibodies and analyzed by flow cytometry.Results The stimulation of T cells with SAM leads to the up-regulation of Fas and FasL. Fas：25.38%±3.17 at 24h,65.11%±3.58 at 96h; FasL：5.23%±1.53 at 24h,20.59%±2.68 at 96h. The blocking of Fas-FasL interaction with anti-Fas monoclonal antibody led to significantly reduce T-cell apoptosis of 20.53%±3.26,while without anti-Fas antibody led to T-cell apoptosis of 48.47%±4.15,but the residual apoptosis activity was higher than that of background remained.Conclusion SAM induces apoptosis of T lymphocytes by the Fas-FasL pathway. Data obtained on the molecular mechanism of apoptosis and the effect was time-dependent.

关 键 词：FAS FASL 细胞凋亡 T淋巴细胞 

分 类 号：R781.4[医药卫生—口腔医学]