乳腺Paget病患者血清标志物的研究  

Study on Serum Biomarkers Screening for Paget's Disease of the Breast

作　　者：石荷玲 杨志波[2] 朱明芳[2] 陈晋广[1] 

机构地区：[1]浙江省台州市立医院妇科,浙江省台州市318000 [2]湖南中医药大学附属第二医院皮肤科

出　　处：《中国肿瘤临床》2010年第9期520-522,共3页Chinese Journal of Clinical Oncology

基　　金：浙江省医药卫生科技计划基金资助(编号:2007B234)~~

摘　　要：目的:目前,蛋白质芯片技术已成为肿瘤蛋白质组学研究的重要工具之一。本研究应用表面增强激光解析电离飞行时间质谱技术(surfaced enhanced laser desorption/ionization time of flight mass spectrometry,SELDI-TOF-MS)寻找乳腺Paget病患者血清特异性蛋白,找出最佳的标志蛋白组合模式作为临床诊断指标,以期用于乳腺Paget病患者的早期诊断。方法:用弱阳离子蛋白芯片(Weak cation exchanger protein chip,WCX2)及SELDI-TOF-MS技术检测10例健康者、10例乳房湿疹和15例乳腺Paget病患者血清中蛋白的相对含量。使用PBSⅡ-C型蛋白质芯片阅读机读取数据,获得的结果采用CIPHERGEN公司的Biomarkerwizard和Biomarker Patterns System软件分析。结果:Paget病患者与健康人相比质荷比为3868Da和8876Da的2个蛋白峰差异有统计学意义(P<0.01);与慢性湿疹相比质荷比为2911Da、3868Da、5097Da的3个蛋白质峰差异有统计学意义(P<0.05)。分析系统筛选出3868Da及8876Da标志蛋白建立起一个乳腺Paget病的诊断模型,对乳腺Paget病的诊断特异性为100%,敏感度为73.3%。结论:SELDI-TOS-MS技术对乳腺Paget病早期诊断和鉴别慢性湿疹具有诊断价值,在特异性肿瘤标记物的筛选等方面有一定应用前景,无创安全,值得推广。Objective： To screen serum biomarkers in patients with Paget＇s disease using surfaced enhanced laser desorption/ionization time of flight mass spectrometry （SELDI-TOF-MS） technique, and to find specific biomarkers which could be used for the early diagnosis of Paget＇s disease. Methods： The serum samples from 15 patients with Paget＇s disease, 10 patients with chronic eczema, and 10 healthy controls were detected with H50 proteinchip and SELDI-TOF-MS. The data were analyzed by Biomarker Patterns Software provided by Ciphergen Corp. A primary diagnosis model of Paget＇s disease was set up, Results： The structure of two proteins （3868Da and 8876Da） were significantly different in healthy people and Paget＇s disease patients （P〈0.01）. Proteins of 2911Da, 3868Da, and 5097Da were significantly different between chronic eczema and Paget＇s disease （P〈0.05）. The M/Z values ranged from 2000Da to 30000Da. The structure of the two proteins （3868Da and 8876Da） was significantly different among the three groups. A diagnostic pattern was established with a sensitivity of 73.3% and specificity of 100%. Conclusion： SELDI-TOF-MS technique is a high-throughput proteomics approach with simple application, high sensitivity and remarkable specificity. SELDI-TOF-MS offers great promise in early diagnosis of Paget＇s disease and screening of tumor biomarkers.

关 键 词：PAGET病 弱阳离子蛋白芯片 表面增强激光解析电离 飞行时间质谱 

分 类 号：R739.5[医药卫生—肿瘤]