循环内皮细胞的检测及其在肿瘤临床中的应用进展  

Circulating endothelial cells:Measurement and clinical application in oncology

作　　者：周鑫(综述)[1] 夏欣一(综述 黄宇烽(审校)[1] 

机构地区：[1]南京军区南京总医院解放军临床检验医学研究所,南京210002

出　　处：《医学研究生学报》2010年第5期543-546,共4页Journal of Medical Postgraduates

基　　金：江苏省科技厅生殖健康研究技术服务平台项目(BM2008151)

摘　　要：循环内皮细胞(circulating endothelial cells,CEC)是由于血管损伤而从血管壁脱落的成熟内皮细胞,其增殖、迁移和黏附形成管腔,在肿瘤新生血管形成过程中发挥重要作用。晚期恶性肿瘤患者CEC数量显著高于正常人对照者,提示CEC可作为抗血管生成药物的靶点和疗效监测的标志物。常用的检测CEC的方法有Hladovec法、Percoll密度梯度快速一步分离法、免疫磁性分离和流式细胞术等。近来流式细胞术发展了6色标记法,成为一种快速、可靠、灵敏的CEC检测方法。由于CEC与肿瘤血管发生密切相关,可在临床用于疗效监测、优化用药剂量、判断预后、评估药物血管毒性等。Circulating endothelial cells（CEC） are mature endothelial cells sloughed off the vessel wall as a result of vascular insults.The proliferation,migration and adhesion of CECs play an important role in the formation of new tumor blood vessels.The significant increase in the number of CECs in patients with advanced malignancies indicates that CECs are possible targets for antiangiogenic therapy and predictors of treatment efficacy.There are four methods to detect CECs,Hladovec,Percoll density gradient centrifugation one-step abstraction,immunomagnetic isolation,and flow cytometry.Recently a six-colour flow cytometric approach was developed,which made flow cytometry a rapid,reliable and sensitive method.CECs,with their pivotal role in tumor angiogenesis,can be applied in monitoring therapeutic efficacy,optimizing drug dose,predicting prognosis,and assessing drug vascular toxicity.

关 键 词：循环内皮细胞 内皮祖细胞 血管发生 流式细胞术 

分 类 号：R730.23[医药卫生—肿瘤]