核因子-κB1-94ins/delATTG基因多态性与中国青岛地区汉族人群急性进展性脑梗死相关性的研究  

Relationship between NF-κB1 gene polymorphism and acute progressive cerebral infarction of Chinese Han population in Qingdao district

作　　者：毛德军[1] 唐咏春[1] 郭瑞友[1] 战淑彩 臧云华[1] 

机构地区：[1]青岛大学医学院附属海慈医院神经内科,青岛266033 [2]青岛市即墨兰村中心卫生院内科,266232

出　　处：《中华神经医学杂志》2010年第5期487-491,共5页Chinese Journal of Neuromedicine

摘　　要：目的 探讨核因子-κB1（NF-κBl）-94ins/delATrG基因多态性与中国青岛地区汉族人群急性进展性脑梗死（APCI）的相关性. 方法 采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）方法 检测100例急性脑梗死患者（ACI组）和99例APCI患者（APCI组）NF-κB1-94ins/如1ATTG基因多态性;采用细胞免疫组化法检测两组患者外周血单个核细胞（PBMC）胞核NF-κBp65表达率的变化. 结果 APCI组TT基因型和T等位基因频率均明显高于ACI组,比较差异有统计学意义（P〈0.05）;等位基因频率的相对风险分析发现T等位基因携带者发生APCI的风险是C等位基因的1.622倍;Logistic回归分析显示.TT基因型与APCI的发病独立相关（OR=2.14,95%CI：2.654～8.296,P〈0.05）.APCI组TT基因型个体PBMC胞核NF-κBp65表达率明显高于ACI组,比较差异有统计学意义（P〈0.05）;Logistic回归分析显示,TT基因型个体PBMC胞核NF-κBp65表达率与APCI的发病独立相关（OR=1.96,95%CI：2.267～7.691,P〈0.05）. 结论 NF-κB1-94ins/delA TTG基因多态性参与了APCI发生.T等位基因可能是中国青岛地区汉族人群APCI发病的遗传易感基因.携带T等位基因的个体可能通过上调NF-κB1的表达而增加APCI的发病风险.Objective To explore the relationship between NF-kB1-94ins/delATTG gene polymorphism and acute progressive cerebral infarction（APCD ofChinese Hart population in Qingdaodistrict Methods We detected the polymorphism of NF-κB1 -94ins/delA TTG gene in 100 patients with acute cerebral infarction （ACI group） and 99 patients with acute progressive cerebral infarction （APCI group） by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）analysis. The changes of expression of NF-κBp65 in PBMC cellular nucleus in the 2 groups were detected by cell immunohistochemistry. Results The frequency of TT genetype and T allele in the APCI group was significantly higher than that in the ACI group （P〈0.05）. Analysis on the relative risk of allele frequency showed that patients with T allele had 1.622 times of risk in having APCI than patients with C allele; logistic regressive analysis indicated that NF-κB1 TT genotype was independently related to the attacking of APCI （OR=2.14, 95% CI： 2.654-8.296, P〈0.05）. The expressions of NF-κBp65 of PBMC cellular nucleus of TT genotypic individuals in APCI group were significantly higher than those in ACI group （P〈0.05）; logistic regressive analysis indicated that the expressions of NF-KBp65 in PBMC cellular nucleus of TT genotypic individuals were independently related to the attacking of APCI （OR=1.96; 95% CI： 2.267-7.691; P〈0.05）. Conclusion The NF-κB1 gene polymorphism might participate in the onset of APCI and T allele of NF-κB1 gene might be a genetic risk factor of getting APCI for Chinese Han populations in Qingdao district. The NF-κB1 T allele carrier might increase the happening of APCI through up regulating the expression of NF-kB1.

关 键 词：急性进展性脑梗死: 核因子-κB1-94ins/delATTG基因 基因多态性 

分 类 号：R743.33[医药卫生—神经病学与精神病学]