不同时间窗经颅磁刺激对脑梗死大鼠Bel-2及脑源性神经营养因子表达的影响  

Transcranial magnetic stimulation and the expression of Bcl-2 and brain derived neurotrophic factor after cerebral infarction

作　　者：李嫚[1] 王梦嵽[1] 宋艳玲[1] 梅元武[1] 黎刚[1] 方瑗[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经内科,武汉430032

出　　处：《中华物理医学与康复杂志》2010年第4期245-248,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：基金项目：国家自然科学基金（NO.30640010）

摘　　要：目的 观察大鼠脑缺血-再灌注后不同时间窗开始经颅磁刺激（TMS）对其B细胞淋巴瘤/白血病-2基因（Bcl-2）及脑源性神经营养因子（BDNF）表达的影响.方法 将100只SD大鼠随机分为正常组、假手术组、磁刺激组及模型组,各组又根据术后干预时间点细分为术后6 h、12 h、24 h、48 h及72 h共5个亚组.采用线栓法将磁刺激组及模型组大鼠制成左侧大脑中动脉栓塞/再灌注（MCAO/R）模型.磁刺激组各亚组分别于脑缺血再灌注后6 h、12 h、24 h、48 h及72 h进行TMS治疗,而正常组、假手术组及模型组各亚组均于上述相同时间点给予假磁刺激.各组大鼠均于治疗14 d后取脑,采用实时荧光定量PCR技术检测脑梗死侧Bcl-2及BDNF mRNA表达情况.结果 各组大鼠梗死侧脑标本均检测到BDNF及Bcl-2 mRNA阳性表达.磁刺激组各亚组Bcl-2及BDNF mRNA表达均显著高于其它各亚组水平（模型组术后72 h亚组除外）;对磁刺激组各亚组进行组内比较发现,该组各亚组BDNF及Bcl-2 mRNA间差异具有统计学意义（P〈0.05）,其中以术后24 h亚组BDNF及术后12 h亚组Bcl-2 mRNA表达水平相对较高.结论 脑缺血再灌注12-24 h内给予TMS治疗,能显著促进脑梗死大鼠BDNF及Bcl-2表达,对保护受损神经细胞、促进神经功能恢复具有重要意义.Objective To study the effect of transcranial magnetic stimulation （TMS） on the rehabilitation of rats with cerebral infarction. Methods One hundred Sprague-Dawley rats were randomly divided into a normal group, a sham-operated control group, a model group and a TMS group with 25 rats in each group. A cerebral infarc- tion model was established in the latter two groups by left middle cerebral artery occlusion （MCAO）. TMS was started at either 12 or 24 hours after reperfusion, and sham-TMS was given to the first two groups at the same time points. The expression of Bcl-2 mRNA and BDNF mRNA were measured by RT-PCR after 14 days. Results Bel-2 mRNA and BDNF mRNA were detected in all groups. The expression of Bcl-2 mRNA in the TMS-12 h group, and that of BDNF mRNA in the TMS-24 h group were significantly higher than in the other groups. Conclusions The expres- sion of Bel-2 mRNA and BDNF mRNA in the brains of rats after cerebral infarction peak when TMS is administered 12 h and 24 h after reperfusion, respectively. TMS might have protective and rehabilitative effects on rats after cere- bral infarct.

关 键 词：经颅磁刺激 脑梗死 细胞淋巴瘤/白血病-2基因 脑源性神经营养因子 

分 类 号：R743.3[医药卫生—神经病学与精神病学]