脱脂程序对异体松质骨免疫原性影响的实验研究  

Influence of defatting procedure on immunogenicity of cancellous bone allograft

作　　者：朱加亮[1] 侯树勋[1] 衷鸿宾[1] 解英俊[1] 

机构地区：[1]解放军总医院第一附属医院骨科实验室全军骨科研究所,北京100048

出　　处：《中国矫形外科杂志》2010年第9期762-767,共6页Orthopedic Journal of China

摘　　要：[目的]研究脱脂程序对异体松质骨免疫原性的影响,并探讨其可能的机制。[方法]采用4种不同程序处理待移植异体骨块,检测处理后各组骨块总脂肪和总蛋白含量。根据待移植骨块的不同将受体大鼠分为4组,行双侧股部肌囊袋内骨块植入。于术后1、2、4、8周取材,检测体外淋巴细胞二次刺激增殖指数、血CD4+/CD8+细胞比值,并进行组织学观察。对术后4周时各组的免疫反应指标同相应骨块总脂肪含量行相关分析。[结果]骨块内总脂肪含量为A组>B组>C组>D组。对移植术后体外淋巴细胞二次刺激增殖指数及血淋巴细胞CD4+/CD8+比值测定发现:同一时间点各组水平均为A组>B组>C组>D组,且均于术后4周时达到峰值。各组骨块总脂肪含量同移植术后4周时体外淋巴细胞二次刺激增殖指数、血淋巴细胞CD4+/CD8+比值呈正相关。组织学观察示移植早期各组骨块周围炎性细胞浸润程度:A组>B组>C组>D组;D组在术后4周时可见血管长入,8周时可见新骨的形成。[结论]脱脂程序可有效降低异体松质骨移植后免疫反应,并可能提高移植后的成骨能力。总脂肪含量可作为提示异体骨移植后免疫反应强弱的一个指标。[Objective]To explore the influence of defatting procedure on immunogenicity of cancellous bone allograft and the possible mechanism. [Methods]Sixty inbred Lewis rats were killed.Distal femoral metaphyses were resected as cancellous bone grafts.Grafts were divided into four groups （A,B,C and D） randomly with four different procedures respectively.Content of total lipid and total protein in grafts were measured.Forty-eight inbred Fisher344 rats were divided into four groups （A,B,C and D） randomly according to different grouped grafts which were transplanted into double femoral muscle pouches of the recipients.Three rats of each group were killed at the 1,2,4 and 8 weeks post operatively respectively.Proliferation index and ratio of CD4＋/CD8＋ were tested and histological changes of transplanted grafts were observed.The correlation analysis was performed between proliferation index,CD4＋/CD8＋ ratio at the 4th post-operative week and the content of total lipid.[Results]Results of measurement showed content of total lipid in four grouped grafts was：groups A BCD and the differences between groups were statistically significant（P〈0.05）.There was no significant difference in protein content between groups（P〉0.05）.Proliferation index increased in the early phase and reached the peak at the 4th post-operative week.Level of proliferation index in four groups was gradiently decreased from group A to group D.At the 4th post-operative week,differences of proliferation index were statistically significant between groups（P〈0.05）,except between group C and group D（P〉0.05）.The changing trend of ratio of CD4＋/CD8＋ was similar to that of proliferation index.Histological observations showed inflammatory cells infiltrated in early phase gradiently decreased from group A to group D.At the 4th post-operative week,histological observations showed neovascularization in group D.At the 8 weeks post-operatively,more new bone formation was observed in group D. The correlation analysis showed signif

关 键 词：同种异体 松质骨 移植 脱脂 免疫原性 

分 类 号：R687.3[医药卫生—骨科学]