尼古丁对胆碱乙酰转移酶在人牙周膜成纤维细胞中表达的影响  

human Periodontal Ligament fibroblasts

作　　者：葛鑫[1] 王勇[1] 刘颍凤[1] 王小竞[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2010年第4期200-203,195,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(30572045)

摘　　要：目的:检测人牙周膜成纤维细胞(human periodontal ligament fibroblasts,hPDLF)中胆碱乙酰转移酶(choline acetyltransferase,ChAT)的表达,并观察尼古丁对其表达的影响,以探讨ChAT在吸烟与牙周炎发生发展过程中的可能作用。方法:体外培养hPDLF,免疫组化和RT-PCR方法检测hPDLF中ChAT表达,并观察尼古丁(10～12mol/L)作用于hPDLF24h后ChAT表达的变化。结果:免疫组化染色hPDLF胞浆中ChAT表达阳性;RT-PCR检测hPDLF中ChATmRNA阳性表达。尼古丁作用后ChAT蛋白和mRNA表达均明显上调与对照组相比差异有统计学意义(P<0.05)。结论:hPDLF中存在非神经元型胆碱能系统物质ChAT的表达,尼古丁可上调其表达。AIM：To examine whether human periodontal ligament fibroblasts （hPDLF） express Choline acetyltransferase （ChAT）,a non-neuronal acetylcholine system substance,and to observe the effects of nicotine on of ChAT in hPDLF.METHODS：Expression of ChAT in hPDLF was detected by immunohistochemistry （IHC） at protein level and by RT-PCR at mRNA level.The effects of nicotine on ChAT was observed by treating hPDLF with nicotine （10-12mol/L） for 24 h.RESULTS：Positive staining of ChAT was detected by IHC in the cytoplasm of hPDLF.mRNA expression of ChAT in hPDLF was confirmed by RT-PCR.Nicotine treatment significantly increased both the mRNA and protein expression of ChAT in hPDLF （P0.05）.CONCLUSION：hPDLF expresses ChAT and nicotine can up-regulate ChAT expression at mRNA and protein levels in hPDLF.

关 键 词：非神经元型胆碱能系统 胆碱乙酰转移酶 人牙周膜细胞 牙周炎 尼古丁 

分 类 号：R780.2[医药卫生—口腔医学]