混合菌对原油的降解及其降解性能的研究  

Biodegradation characteristics of crude oil by mixed bacterial strains

作　　者：何丽媛[1] 党志[1,2] 唐霞[1] 卢桂宁[1] 郭楚玲[1,2] 吴平霄[1,2] 刘从强[3] 

机构地区：[1]华南理工大学环境科学与工程学院,广州510006 [2]工业聚集区污染控制与生态修复教育部重点实验室,广州510006 [3]中国科学院地球化学研究所、环境地球化学国家重点实验室,贵阳550002

出　　处：《环境科学学报》2010年第6期1220-1227,共8页Acta Scientiae Circumstantiae

基　　金：广东省自然科学基金研究团队项目(No.9351064101000001);广东省自然科学基金重点项目(No.05103552);广东省科技计划项目(No.2007A050100023);环境地球化学国家重点实验室开放基金~~

摘　　要：从污染土壤中分离筛选到四株石油组分降解菌被用于组建降解原油的混合菌体系.石油组分降解菌包括:烷烃降解菌洋葱伯克霍尔德氏菌(Burkholderia cepacia)GS3C、菲降解菌鞘氨醇单胞菌(Sphingomonas sp.)GY2B、芘降解菌GP3(假单胞菌(Pseudomonas sp.)GP3A和伯克菌科的菌株(Pandoraea pnomenusa)GP3B).气相色谱-质谱联用法(GC-MS)用于对原油降解性能的测定,并对原油组分的降解情况进行详细分析.通过对不同菌株的混合培养比较,得到降解原油的最佳组合G8(GS3C+GY2B+GP3B),培养5d后使初始原油浓度为2000mg·mL-1的总去除率达到69.20%,并且对烷烃类和芳烃类化合物都表现出较强的降解能力.混合菌G8对原油的总去除率比单菌提高了近30%,其最适生长条件为:温度为30℃,初始pH值为7,接种量为4%.Four oil-degrading bacteria strains,isolated from oil-contaminated soil,were mixed to construct different bacterial consortiums for crude oil degradation.The bacterial strains included the pyrene-degrading strains Pseudomonas GP3A and Pandoraea Pnomenusa GP3B,the phenanthrene-degrading strain Sphingomonas GY2B,and the cetane-degrading strain Burkholderia cepacia GS3C.GC-MS was used to analyze the degradation products.The results indicated that combination G8（GS3C＋GY2B＋GP3B）showed optimal degradation ability toward both aliphatic and aromatic hydrocarbons.The removal efficiency by combination G8 reached 69.20% in 5 days when the initial crude oil concentration was 2000 mg·L^-1,which was 30% higher than that of the individual bacterial strains.The optimum conditions for G8 growth were 30℃,initial pH 7,and 4% inoculation volume.

关 键 词：混合菌 原油 生物降解 GC-MS 

分 类 号：X172[环境科学与工程—环境科学]