抗霉素产生菌的诱变育种及发酵研究  

Studies on mutagensis and fermentation of antimycin producing strain

作　　者：林宇涵 王怀旺[1] 洪秀清[1] 林永勉[2] 郑卫[1,2,3] 

机构地区：[1]福建医科大学药学院,福州350004 [2]福建省微生物研究所,福州350007 [3]福建省新药(微生物)筛选重点实验室,福州350007

出　　处：《中国抗生素杂志》2010年第5期349-352,共4页Chinese Journal of Antibiotics

摘　　要：抗霉素产生菌Streptomyces sp.FIM-04114,经紫外-氯化锂复合处理,筛选得到一株高产菌No.1-095,其抗霉素摇瓶发酵效价为170mg/L,且遗传稳定性良好。经发酵过程工艺优化,菌株No.1-095摇瓶发酵效价达220mg/L,较出发菌株FIM-04114提高了63.0%。在50L自动发酵罐上发酵,抗霉素效价为206mg/L。A mutant strain No. 1-095 with higher potency（170mg/L） and better hereditary stability was obtained by combination treatment of UV and LiCl from antimycin producing strain FIM-04114.After the fermentation conditions of strain No. 1-095 in shake flask were optimized, the antimycin yield of the strain was 220mg/L, which was 63.0% higher than the original strain FIM-04114.The potency of strain No. 1-095 was 206mg/L in a 50L bioreactor.

关 键 词：抗霉素 诱变育种 发酵 

分 类 号：TQ465[化学工程—制药化工]