高效液相色谱法测定猪肉中四环素类抗生素及其代谢产物  

Tetracyclines and their metabolites in pork by HPLC

作　　者：雒婉霞[1] 赵清荣[1] 朱芸[1] 蔡增轩[2] 杨勤德[1] 

机构地区：[1]新疆维吾尔自治区疾病预防控制中心,乌鲁木齐830001 [2]浙江省疾病预防控制中心,杭州310000

出　　处：《中国卫生检验杂志》2010年第5期1018-1020,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立猪肉中7种四环素类抗生素及3种代谢产物多残留的高效液相色谱紫外检测器同时测定方法。方法:样品经pH4.0的EDTA-Mcllvaine缓冲液提取,提取液用HLB固相萃取柱净化。在Waters Atlantis T3(4.6×250 mm,5μm)色谱柱上,以甲醇:乙腈(2:3)-0.2%甲酸溶液为流动相(梯度洗脱),流速1.0 ml/min,采用350 nm紫外光进行检测,在35 min内将7种四环素类抗生素及其3种代谢产物全部洗脱并达到基线分离。结果:方法检出限:4.5μg/kg～22μg/kg,定量下限:25μg/kg～80μg/kg,线性范围:25μg/kg～1250μg/kg,加标回收率:82.5%～101.9%,相对标准偏差:0.8%～8.3%。结论:本方法具有简便、快速、准确的优点,适用于猪肉中7种四环素及其3种代谢产物的同时检测。Objective：A high performance liquid chromatographic method was developed for the determination of tetracyclines and their metabolites in pork.Methods：The samples were extracted with EDTA-Mcllvaine buffer group（pH4.0） and were purified by HLB solid phase extraction column.TCs were successfully separated on a Waters Atlantis T3（4.6×250 mm,5 μm） column using Methyl alcohol-Methyl cyanide-0.02% Formic acid solution as mobile phase at a flow rate of 1.0 ml/min and detected at 355 nm with 35 min.Results：The detection limit of the method was 4.5 μg/kg～22 μg/kg and the quantitative lower limit was 25 μg/kg～80 μg/kg.The linear range is from 25 μg/kg to 1250 μg/kg.Overall recoveries were between 82.5% and 101.9%.RSD value was 0.8%～8.3%.Conclusion：The methods simple,rapid and accurate.It can be used for the determination of tetracyclines and their metabolites in pork.

关 键 词：四环素 高效液相 测定 猪肉 

分 类 号：O657.72[理学—分析化学]