瘦素受体基因Pro1019 Pro变异与温州地区胃癌的相关性研究  

Study on relationship between variation of Pro1019 Pro in leptin receptor gene and gastric cancer of "Han" population in Wenzhou

作　　者：应俊[1] 应俊林[2] 李佩珍[1] 姚蔚[1] 张洪勤[1] 

机构地区：[1]温州医学院生物学实验教学中心,浙江温州325000 [2]温州大学化学与材料工程学院,浙江温州325000

出　　处：《中国卫生检验杂志》2010年第5期1135-1136,共2页Chinese Journal of Health Laboratory Technology

基　　金：浙江省教育厅科技项目(20061788)

摘　　要：目的:研究温州地区胃癌与瘦素受体Pro1019 Pro基因变异的关系。方法:运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定无亲缘关系的221例温州地区汉人的瘦素受体基因Pro1019 Pro的基因型(其中胃癌组117例,正常对照组104例),并分析Pro1019 Pro基因多态性与糖尿病的关系。结果:①胃癌组Pro1019 Pro基因型GG、GA及AA的频率分别为0.017、0.205和0.778;G和A等位基因频率分别为0.120和0.880;正常对照组基因型GG、GA及AA的频率分别为0.077、0.365和0.558;G和A等位基因频率分别为0.260和0.740;②胃癌组A、G等位基因分布频率与正常对照组比较差异有显著性(P<0.05)。结论:温州地区汉族人群存在瘦素受体基因Pro1019 Pro变异,它可能是该地区人群胃癌患者的遗传易感标记,A等位基因与胃癌发病有一定关系。Objective：To investigate the association between the variation of Pro1019Pro in leptin receptor gene and gastric cancer of ＂Han＂ population in Wenzhou areas.Methods：The genotypes of Pro1019Pro variation in 221 unrelated subjects of ＂Han＂ population in Wenzhou（including 117 cases of gastric cancer and 104 controls）were tested by polymerase chain reaction-restriction fragment length polymorphisms（PCR-RFLP）.Results：In patients of gastric cancer the frequencies of genotype GG,GA and AA were 0.017,0.205 and 0.778 respectively,while in control they were 0.077,0.365 and 0.558 respectively.The frequency of allele A in gastric cancer was 0.880,which was significantly higher than that in controls（P〈0.05）.The frequency of allele G in patients of gastric cancer was 0.120,which was significantly lower than that in controls（0.260）（P〈0.05）.Conclusion：The variation of Pro1019Pro in LEPR gene exists in ＂Han＂ population in Wenzhou areas,which may be a genetic marker of gastric cancer.The allele A is related with the pathogenesis of gastric cancer.

关 键 词：瘦素受体基因 基因多态性 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]