韶关市2008年B型流感病毒流行及基因变异分析  

Analysis of epidemic of influenza B virus and mutation of hemagglutinin HA1 in 2008 in Shaoguan

作　　者：陈艳[1] 张欣[2] 邓俊兴 倪汉忠[2] 胡国超[1] 宾羽琳[1] 邱灿林[1] 钟启丽 

机构地区：[1]广东省韶关市疾病预防控制中心,广东韶关512028 [2]广东省疾病预防控制中心,广州510300

出　　处：《中国卫生检验杂志》2010年第5期1161-1162,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:了解2008年韶关市B型流感病毒的流行及基因变异情况。方法:收集2008年韶关市流感监测及疫情资料,选取流感疫情中分离的B型毒株,提取病毒RNA,RT-PCR扩增病毒HA1基因,纯化产物进行核苷酸序列测定,使用DNA Start MegAlign等生物软件包进行序列分析。结果:2008年流感监测与疫情中一共分离到39株B型流感毒株,分离率为7.39%(39/528),与B型流感病毒相关的疫情达到当年疫情数量的一半(7/14),有4起完全由B型流感病毒引起,有3起同时检出B型流感病毒与A型流感病毒,爆发时间多集中在3月;B/SG/y010/2008与B/Shanghai/361/2002相比,3个氨基酸替换发生在抗原决定簇,与韶关以往毒株B/SG/173/1999比较4个氨基酸替换发生在抗原决定簇,均在196增加了一个糖基化位点。结论:B型流感病毒是韶关市2008年流感流行优势毒株,B/SG/y010/2008与B/Shanghai/361/2002相比发生了一定的变异,而与韶关以往毒株B/SG/173/1999相比则发生了抗原性漂移。Objective：To understand the epidemic of influenza B virus and the mutation of hemagglutinin HA1 in Shaoguan in 2008.Methods：Analyze the data of influenza surveillance and outbreaks in Shaoguan,choose influenza B virus strain from outbreaks,extract virion RNA and RT-PCR which were purified and tested for nucleic acid sequences.Then compare and analyze the sequences using DNA Start MegAlign software.Results：39 Influenza B Virus were isolated in 2008,isolated rate was 7.39%（39/528）;50% of influenza outbreaks correlated with Influenza B Virus（7/14）,4 were Influenza B Virus only,3 were Influenza A and B Virus;March appeared as the epidemic peak time.Comparing B/SG/y010/2008 with B/Shanghai/361/2002 and B/SG/173/1999,3 and 4 amino acid replacements were discovered in antigenic determinant respectively,one glycosylation raised at 196.Conclusion：The predominant influenza virus is influenza B virus in 2008.Comparing B/SG/y010/2008 with B/Shanghai/361/2002,the mutation of influenza B virus had occurred,and antigenic drift occurred between B/SG/y010/2008 and B/SG/173/1999.

关 键 词：B型流感病毒 流行 基因变异 

分 类 号：R725[医药卫生—儿科]