细针直接穿刺枕大池二次注入自体血致犬脑血管痉挛模型的建立  

Experimental cerebral vasospasm induced by injecting autologous blood into cisterna magna twice after puncture of cisterna magna with a fine needle in dogs

作　　者：陈红生[1] 王志萍[2] 

机构地区：[1]江苏省麻醉学重点实验室江苏省麻醉医学研究所,徐州市221002 [2]徐州医学院附属医院麻醉科

出　　处：《中华麻醉学杂志》2010年第3期362-364,共3页Chinese Journal of Anesthesiology

基　　金：江苏省卫生厅基金（H200723）;江苏省医学重点人才资助项目（RC2007093）;江苏省高校自然科学重大基础研究项目（08KJA320001）

摘　　要：目的 评价采用细针直接穿刺枕大池二次注入自体血致犬脑血管痉挛模型建立的可行性.方法 成年健康杂种犬6只,雌雄不拘,体重14～ 18 kg.腹腔注射3%戊巴比妥钠30～40 mg/kg麻醉下,保留自主呼吸.用细针穿刺枕大池后,缓慢放出脑脊液0.3 ml/kg,然后注入自体股动脉血0.5 ml/kg,速率0.5 ml/s;48 h后再次注入等容量自体股动脉血.于第1次注血前（T1）、第2次注血后7 d（T2）、14 d（T3）和21 d（T4）时,行神经功能评分,并进行CT血管造影,测量基底动脉直径.结果 术中和术后无一只犬死亡.与T1时比较,T2.3时神经功能评分升高,基底动脉直径缩短（P＜0.05）,T4时神经功能评分和基底动脉直径差异无统计学意义（P＞0.05）.结论 采用细针直接穿刺枕大池二次注入自体血可成功建立犬蛛网膜下腔出血后脑血管痉挛模型,术后生存率高,对动物损伤小.Objective To investigate the feasibility and effectiveness of inducing cerebral vasuspasm by injecting autologous blood into cisterna magna （CM） twice after puncture of CM with a fine needle in dogs.Methods Six healthy aduh mongrel dogs of both sexes weighing 14-18 kg were involved in this study. The animals were anesthetized with intraperituneal 3% pentobarbital sodium 30-40 mg/kg. Spontaneous breathing was kept. Nonheparinized autologous arterial blood 0.5 ml/kg was injected into CM twice at the rate of 0.5 ml/s at 48 h interval after puncture of CM with a fine needle. Neurological function was assessed and scored （ 1 = normal, 5 =dead） and CT angiography was performed to determine the diameter of cerebral basilar artery before the first CM injection （T1 , baseline） and at days 7, 14 and 21 after the second CM injection （T2.4 ）. Results The injection of autologous blood into CM twice significantly increased the success rate of the puncture and intraoporative survival rote of the animal＊. The neurological function scores were significantly increased and diameter of cerebral basilar artery shortened at T2 and T3 compared with the baseline value. Conclusion The model of cerebral vasospasm after subarachnoid hemorrhage can be successfully established by injecting autologous blood into CM twice following puncture of CM with a fine needle in dogs, with higher postoperative survival rate and less injury to the animals.

关 键 词：注射 脑室内 输血 自体 血管痉挛 颅内 

分 类 号：R743.3[医药卫生—神经病学与精神病学]