菌源处理方式对发酵产氢微生物菌群及产氢性能的影响  

Effect of treatment methods for natural bacteria source on microbial community and bio-hydrogen-producing potential

作　　者：刘义[1] 樊绍群[2] 徐耀伟[1] 樊耀亭[1] 侯红卫[1] 

机构地区：[1]郑州大学化学系,河南郑州450052 [2]中国电信股份有限公司北京研究院,北京100035

出　　处：《化工学报》2010年第6期1545-1549,共5页CIESC Journal

基　　金：国家重点基础研究发展计划项目(2009CB220005);国家自然科学基金项目(20871106; 90610001);高校博士点专项科研基金项目(20070459007);教育部郑州大学'211'工程项目~~

摘　　要：The pretreatment of bacteria source is one of the key factors affecting the efficiency of bio-hydrogen production.Prior to inoculation,cow dung compost as the natural microbial consortium is treated by following methods:(1)forced aeration combined adding sugar after infrared drying;(2)infrared drying;(3)infrared drying combined forced aeration;and (4)water immersion.The effects of the microbial community on bio-hydrogen production were investigated in a 20 L continuous stirred tank reactor (CSTR).The results showed that the maximum hydrogen production ability,hydrogen content and the rate of hydrogen production was 387.6 ml H2·(g-sucrose)-1,55.5% and 0.5089 L H2·(L·h)-1 at fixed sucrose concentration of 10 g·L-1,operating pH of 5.0±0.1 and the pretreated bacteria source by method (1),respectively.The contents of acetic acid and butyric acid were more than 75% of VFAs in the terminal liquid phase by-product.Furthermore,the image analysis by PCR-DGGE technology showed that there was district difference in the number of electrophoresis band and the strength.The sample,of which the bacteria source was treated by method (1),exhibited two maximum illumination bands,which implies that there exist two kinds of dominant microbial community.The pretreatment of bacteria source is one of the key factors affecting the efficiency of bio-hydrogen production.Prior to inoculation,cow dung compost as the natural microbial consortium is treated by following methods：（1）forced aeration combined adding sugar after infrared drying;（2）infrared drying;（3）infrared drying combined forced aeration;and （4）water immersion.The effects of the microbial community on bio-hydrogen production were investigated in a 20 L continuous stirred tank reactor （CSTR）.The results showed that the maximum hydrogen production ability,hydrogen content and the rate of hydrogen production was 387.6 ml H2·（g-sucrose）-1,55.5% and 0.5089 L H2·（L·h）-1 at fixed sucrose concentration of 10 g·L-1,operating pH of 5.0±0.1 and the pretreated bacteria source by method （1）,respectively.The contents of acetic acid and butyric acid were more than 75% of VFAs in the terminal liquid phase by-product.Furthermore,the image analysis by PCR-DGGE technology showed that there was district difference in the number of electrophoresis band and the strength.The sample,of which the bacteria source was treated by method （1）,exhibited two maximum illumination bands,which implies that there exist two kinds of dominant microbial community.

关 键 词：生物制氢 菌源处理方式 凝胶电泳分析 发酵 

分 类 号：TK91[动力工程及工程热物理] X712[环境科学与工程—环境工程]