大鼠脑出血后的细胞凋亡及其与NF-κB和I-κBα蛋白表达的关系  

The relationship between apoptosis and the expression of NF-κB and I-κBα after intracerebral hemorrhage in rats

作　　者：张莲香[1] 马全瑞[1] 李军平[1] 文玉军[1] 张海宇[1] 秦毅[1] 

机构地区：[1]宁夏医科大学人体解剖学与组织胚胎学系,银川750004

出　　处：《神经解剖学杂志》2010年第1期58-63,共6页Chinese Journal of Neuroanatomy

基　　金：宁夏教育厅基金(NJ2006;NJ2008);宁夏医科大学重点项目(NY2009)

摘　　要：目的:探讨大鼠脑出血(ICH)后细胞凋亡及其与NF-κB和I-κBα蛋白表达的关系和演变规律,本实验用Ⅶ胶原酶联合肝素注入大鼠右侧脑纹状体制备脑出血模型。方法:TUNEL染色(末端脱氧核糖核苷转移酶介导的dUTP缺口末端标记法)观察细胞凋亡的变化;免疫组化SABC法检测NF-κB和I-κBα蛋白的表达;Western Blot技术检测NF-κB和I-κBα蛋白相对光密度值。结果:(1)ICH组TUNEL阳性细胞于出血后12h可检测到,3d达高峰,7d仍有较高表达(P<0.01);(2)ICH组I-κBα和NF-κB蛋白免疫阳性细胞分别于出血12h、3d达高峰,7d仍高于假手术组和正常对照组(P<0.01);(3)Western Blot结果显示NF-κB相对光密度比值在ICH3d出血侧达高峰,I-κBα蛋白相对光密度比值在ICH组ICH侧12h达高峰,7d时仍明显高于正常对照组(P<0.05)。结论:ICH后存在细胞凋亡;NF-κB和I-κBα参与了ICH所致的细胞凋亡。Objective：In order to investigate the relationship between apoptosis and the expression of NF-κB and I-κBα protein after intracerebral hemorrhage（ICH）in rats.Methods：The ICH model was induced by injection of Ⅶ bacterial collagenase mixed with heparin into the right caudate nucleus of the rats.The changes of cell apoptosis were observed by terminal deoxynucleotidyl transferease mediated dUTP nick end-labeling（TUNEL）;the expression of NF-κB and I-κBα proteins were detected by immunohistochemical SABC method.The relative optical density of NF-κB and I-κBα proteins were measured by Western Blot.Results：① TUNEL positive cells appeared at 12 h after ICH,it peaked at 3 d,and it maintains the high expression at 7 d（P〈0.01）;② The number of I-κBα and NF-κB positive cells peaked at 12 h and 3 d after ICH.At 7 d,it still higher than that of SCG and NCG（P〈0.01）;③ The results of Western-blot showed that the relative optical density of NF-κB and I-κBα proteins were most highest in ICH side 3 d and 12 h respectively after ICH.The relative optical density of NF-κB and I-κBα in ICH side were also higher than that of normal control group at 7 d（P〈0.05）.Conclusion：The results indicate that apoptotic injury occurs after ICH,NF-κB and I-κBα involved in the cell apoptosis caused by ICH.

关 键 词：脑出血 细胞凋亡 NF-ΚB I-ΚBΑ 大鼠 SABC WESTERN BLOT 

分 类 号：R743.3[医药卫生—神经病学与精神病学]