BAG-1及NF-κBP65在多发性骨髓瘤患者骨髓单个核细胞中的表达  

Expressions of BAG-1 and NF-κB P65 in the mononuclearcell of multiple myeloma patients

作　　者：黄来全[1] 韦中玲[1] 张军[1] 张岱云[1] 汪兴洪[1] 

机构地区：[1]安徽皖南医学院附属弋矶山医院血液内科

出　　处：《中国临床药理学与治疗学》2010年第3期292-297,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：安徽省卫生厅自筹项目(2008B045)

摘　　要：目的:通过观察核酸水平Bcl-2结合抗凋亡基因1(Bcl-2-Associated Athanogene1,BAG-1)及NF-κB P65在多发性骨髓瘤(multiple mye-loma,MM)的表达,探讨BAG-1及NF-κB P65在MM发病机制中的作用及其临床意义,为MM的治疗寻找相关的理论依据。方法:选取皖南医学院附属弋矶山医院2006年10月-2008年10月初诊MM患者为实验组,同时选取骨外伤患者为对照组,抽取患者骨髓液提取单个核细胞,用于BAG-1及NF-κB P65mRNA RT-PCR检测。结果:初诊MM患者BAG-1mRNA在单个核细胞中表达率为71.4%,高于对照组(P=0.007)。NF-κB P65mRNA在初诊骨髓瘤患者单个核细胞中表达率为75.0%,高于对照组(P=0.004)。BAG-1mRNA与NF-κB P65mRNA呈正相关。BAG-1及NF-κB P65高表达的MM患者肿瘤负荷高、与多项临床指标有关。结论:BAG-1及NF-κB P65在MM患者单个核细胞中高表达,与对照组相比差异有统计学意义,与临床多项指标有关,经分析可能参与疾病的发生发展,可作为预后参考指标之一。AIM： To investigate the expressions of BAG-1 and NF-κB P65 in the multiple myeloma （MM） and approach the effects of BAG 1 and NF-κB P65 in the pathogenesis of MM and their clinical significance, which would be useful to find a theoretical basis for the treatment of MM. METHODS： The patients diagnosed MM in Yijishan hospital of wannan medical college from 2006. 10 to 2008. 10 as the experimental group, while the bone trauma patients selected as the control group. The mononuclearcells of bone marrow in the patients were extracted and the BAG-1 and NF-κB P65 mRNA RT-PCR were detected. RESULTS： The expression of BAG-1 mRNA （71.4%）in the MM patients was higher than that in the control group（P=0. 007）. The expression of NF-κB P65 mRNA （75.0%）in the MM patients was higher than that in the control group（P=0. 004）. There was a positive correlation between the BAG-1 and NF-κB P65 mRNA. The tumor load was high in the MM patients with overexpression of BAG-1 and NF-κB P65, which was ralated to a number of clinical parameters indexes. BAG-1 and NF-κB P65 were positive expressed of MM tumor load, relatived with a number of clinical indicators. CONCLUSION. The expressions of BAG-1 and NF-κB P65 in MM are high, there are no difference between the experimental group and control group. BAG- 1 and NF-κB P65 were related to a number of clinical indexes. BAG-1 and NF-κB P65 may be involved in the occurrence and the development of disease, and they can be used as one of the prognosis indexes.

关 键 词：BAG-1 NF-ΚB P65 多发性骨髓瘤 RT—PCR 

分 类 号：R733.3[医药卫生—肿瘤]