L-精氨酸在人肝癌裸鼠肝脏移植瘤生长中的作用  

Effects of L-arginine on tumor growth of human hepatocellular carcinoma in nude mice

作　　者：张剑[1] 李华[1] 汪根树[1] 姜楠[1] 杨扬[1] 陈规划[1] 

机构地区：[1]中山大学附属第三医院肝脏移植中心,广州510630

出　　处：《中华肝胆外科杂志》2010年第4期283-285,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：国家重点基础研究发展计划（973计划）资助项目（2003CB515500）;广东省科技计划资助项目（20088030301050）;国家自然科学基金资助项目（30571769）

摘　　要：目的 探讨L-精氨酸（L-arg）在人肝癌裸鼠肝脏移植瘤生长中的作用.方法 建立人肝癌裸鼠肝脏移植瘤模型,使用L-arg进行干预治疗,采用免疫组化方法 和图像分析技术检测增殖细胞核抗原（PCNA）的表达,原位末端标记（TUNEL）法检测肿瘤细胞的凋亡情况.结果 （1）小剂量L-arg（0.5 g·kg^-1·d^-1）组、大剂量L-arg（1g·kg-1·d^-1））组和对照组移植瘤重量分别为（985±76）mg、（328±35）mg、（586±56）mg;大剂量L-arg组移植瘤重量较对照组显著减少（P＜0.05）;小剂量L-arg组移植瘤重量较对照组显著增加（P＜0.05）.（2）大剂量L-arg组移植瘤PCNA的表达较对照组显著下调（P＜0.05）,小剂量L-arg组和对照组比较无明显差异（P＞0.05）.（3）大剂量L-arg组NO水平较对照组和小剂量组显著升高（P＜0.05）,小剂量L-arg较对照组显著升高（P＜0.05）.（4）大剂量L-arg组移植瘤凋亡指数（AI）明显高于对照组（P＜0.05）;小剂量L-arg对移植瘤AI无明显影响（P＞0.05）.结论 L-精氨酸对肝癌具有双重作用.小剂量L-精氨酸可促进肝癌生长;大剂量L-精氨酸抑制肝癌的生长.Objective To study the effects of L-arginine（L-arg）on tumor growth of human hepatocellular carcinoma（HCC）in nude mice.Methods Tumor tissues of HCC were implanted into the liver of nude mice.Then,nude mice were treated with L-arginine.Immunohistochemical staining and image analysis were used to detect the protein expression of proliferating cell nuclear antigen（PCNA）.Tumor apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling（TUNEL）assay.Results（1）The tumor weight was（985±76）mg,（328±35）mg and（586±56）mg in L-arg（0.5 g·kg^（-1）·d^（-1））,L-arg^（-1） g·kg^（-1）·d^（-1））and control group respectively.The tumor weight was significantly decreased in L-arg（1 g·kg^（-1）·d^（-1） group and significantly increased in L-arg（0.5 g·kg^（-1）·d^（-1））group as compared with the control group（P〈0.05）.（2）The protein expression of PCNA was remarkably down-regulated in L-arg（1 g·kg^（-1）·d^（-1））group than in the control group（P〈0.05）and were not remarkably different between L-arg（0.5 g·kg^（-1）·d^（-1））group and the control（P〉0.05）.（3）Serum nitric oxide（NO）concentration was significantly increased in L-arg（1 g·kg^（-1）·d^（-1））group as compared with L-arg（0.5 g·kg^（-1）·d^（-1））group（P〈0.05）,significantly in-creased in L-arg（0.5 g·kg^（-1）·d^（-1））group as compared with control group（P〈0.05）.（4）Comparing with the control,the apoptosis index of tumor cell was significantly higher in L-arg（1g·kg^（-1）·d^（-1））group （P〈0.05）,and there was no difference in L-arg（0.5 g·kg^（-1）·d^（-1））group（P〉0.05）.Conclusion L-arginine may have a dual pro-and anti-tumor action on HCC.When it is at high concentrations,L-arginine has antitumor activity.It may promote the growth of HCC at a low concentration.

关 键 词：癌 肝细胞 L-精氨酸 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]