PVAX-MAGE-1基因疫苗延缓小鼠肝癌细胞H22腹水瘤和实体瘤的生长  

Growth Inhibition of Mouse Ascites and Solid Hepatoma 22 With PVAX-MAGE-1 Gene Vaccine

作　　者：杨晓玲[1] 吕婧[1] 王建华[2] 张悦红[1] 牛勃[1,2] 

机构地区：[1]山西医科大学生物化学与分子生物学系,太原030001 [2]北京首都儿科研究所生物技术室,北京100020

出　　处：《中国生物化学与分子生物学报》2010年第5期472-477,共6页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：为观察重组基因疫苗PVAX-MAGE-1的抑瘤效应,构建黑色素瘤抗原-1(melanoma antigen-1,MAGE-1)真核基因表达载体--PVAX-MAGE-1.以重组质粒免疫C57BL/6小鼠后,ELISA法检测表明,与对照鼠(PVAX-1和生理盐水注射小鼠)比较,免疫小鼠脾淋巴细胞上清液中的细胞因子IL-2和IFN-γ明显升高(P<0.05);淋巴细胞-肿瘤细胞混合培养证明,免疫小鼠外周血CD8+T细胞对靶细胞的特异性杀伤作用明显增强(P<0.05).体内实验证明,PVAX-MAGE-1免疫C57BL/6小鼠,可显著延缓移植性H22腹水瘤及实体瘤在小鼠体内的生长.实验结果提示,重组基因疫苗PVAX-MAGE-1有明显的延缓肿瘤生长的作用,其抑瘤作用与提高T淋巴细胞IL和IFN表达,增强对肿瘤杀伤作用直接相关.The melanoma antigen-1（ MAGE-1） gene was inserted into a mammalian expression vector PVAX-1 and used to vaccinated C57BL / 6 mice using saline as the controls. The splenocyte-secreted IL-2 and IFN-γ were significantly higher than those of the controls （P〈0. 05）,as well as the CTL activity of CD8 ＋ T cells （ P〈0. 05 ）. In vivo experiments showed that the immunized C57BL /6 mice could inhibit the growth of the transplanted H22 ascites and solid tumor. Our results indicated that PVAX-MAGE-1 gene vaccine was able to suppress the tumor growth,and might involve the enhanced expression of IL and IFN.

关 键 词：肝细胞癌 黑色素瘤抗原-1 基因疫苗 

分 类 号：Q78[生物学—分子生物学] R735[医药卫生—肿瘤]