姜黄素对大鼠肝缺血再灌注早期肝组织一氧化氮表达的影响  

Curcumin reduces hepatic nitric oxide production in earlystage ischemia/reperfusion in rats

作　　者：向进见[1] 田夫[1] 李文岗[2] 李明忠[1] 蒋雪峰[1] 程本静[1] 

机构地区：[1]长江大学附属第一医院普通外科3病区,湖北省荆州市434000 [2]福建医科大学附属厦门第一医院肝胆胰血管外科,福建省厦门市361000

出　　处：《世界华人消化杂志》2010年第10期987-992,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨姜黄素对大鼠肝脏缺血再灌注早期损伤微循环的影响.方法:将大鼠随机分为假手术组、对照组和实验组(姜黄素40mg/kg,2次给药).通过检测再灌注早期1、3h血清转氨酶水平、肝组织中一氧化氮(nitricoxide,NO)、一氧化氮合酶(nitricoxide synthase,NOS),诱导型一氧化氮合酶(inducible nitricoxide synthase,iNOS)mRNA及内皮型一氧化氮合酶(endothelium nitricoxide synthase,eNOS)mRNA水平,以及肝组织病理学检查来评价姜黄素对大鼠肝脏缺血再灌注早期损伤微循环的影响.结果:相对于对照组,姜黄素可降低大鼠肝脏缺血再灌注早期损伤1、3h血清谷丙转氨酶(ALT)的水平(603.8U/L±64.5U/Lvs758.1U/L±114.7U/L,837.1U/L±33.3U/Lvs1012.7U/L±119.8U/L,均P<0.01)和谷草转氨酶(AST)的水平(605.7U/L±65.7U/Lvs779.5U/L±124.3U/L,849.6U/L±36.0U/Lvs1027.8U/L±139.8U/L,均P<0.01);改善肝组织病理学损害;减少肝脏缺血再灌注早期损伤1、3h肝组织由iNOS产生的NO蛋白水平(0.455±0.056vs0.594±0.087,0.492±0.040vs0.671±0.079,均P<0.01);降低肝脏缺血再灌注早期损伤1、3h肝组织iNOSmRNA的表达强度(0.426±0.075vs0.569±0.073,0.527±0.066vs0.702±0.089,均P<0.01).结论:姜黄素可通过减轻肝组织中由iNOS产生的NO生成,来改善肝缺血再灌注早期损伤中微循环的紊乱,从而减少对肝缺血再灌注肝实质细胞的损伤.AIM：To investigate the effects of curcumin on the microcirculation in early-stage ischemia/reperfusion injury in rats.METHODS：Wistar rats were randomized into three groups：sham-operation group,dimethyl sulfoxide （DMSO） group （treated with 1 mL of DMSO twice） and curcumin group （treated with 40 mg/kg of curcumin twice）.The level of serum transaminase,the content of hepatic nitric oxide （NO）,and the expression of nitric oxide synthase （NOS）,inducible nitric oxide synthase （iNOS）and endothelial nitric oxide synthase （eNOS mRNAs in liver tissue were determined to evalu ate the effects of curcumin on the microcirculatio in early-stage ischemia/reperfusion （reperfusio for 1 h and 3 h）.RESULTS：Compared with the DMSO group curcumin decreased the levels of serum alanin transaminase （603.8 U/L ± 64.5 U/L vs 758.1 U/L ± 114.7 U/L and 837.1 U/L ± 33.3 U/L vs 1012.U/L ± 119.8 U/L,respectively;both P〈0.01） and aspartate aminotransferase （605.7 U/L ± 65.7 U/L vs 779.5 U/L ± 124.3 U/L and 849.6 U/L ± 36.U/L vs 1027.8 U/L ± 139.8 U/L,respectively both P〈0.01）,relieved hepatic pathologica damage,reduced the content of NO （0.455 ± 0.05 vs 0.594 ± 0.087 and 0.492 ± 0.040 vs 0.671 ± 0.079 respectively;both P〈0.01） derived from iNOS and down-regulated the expression of iNO mRNA （0.426 ± 0.075 vs 0.569 ± 0.073 and 0.527 0.066 vs 0.702 ± 0.089,respectively;both P〈0.01 in the liver tissue in early-stage ischemia/reper fusion （reperfusion for 1 h and 3 h）.CONCLUSION：Curcumin can relieve hepati cell injury by decreasing the content of NO de rived from iNOS and reducing hepatic microcir culation disturbance in early-stage ischemia/re perfusion injury in rats.

关 键 词：姜黄素 一氧化氮 大鼠 再灌注损伤 微循环 

分 类 号：R743.31[医药卫生—神经病学与精神病学]