兔VX2肝癌肝动脉栓塞术后肿瘤新生血管的变化与分析  

Neovascularization in survival cancerous tissue after transcatheter arterial chemoembolization in rabbits bearing VX2 liver tumor

作　　者：鲁东[1] 吕维富[1] 高斌[2] 张正峰[1] 

机构地区：[1]安徽省立医院影像科,安徽合肥230001 [2]合肥市第一人民医院影像科,安徽合肥230061

出　　处：《中国介入影像与治疗学》2010年第3期305-308,共4页Chinese Journal of Interventional Imaging and Therapy

基　　金：安徽省卫生厅医学科研课题计划(09C222)

摘　　要：目的分析兔VX2肝癌肝动脉碘化油栓塞术后残癌组织内新生血管生成的变化。方法将36只荷VX2肝癌实验兔随机分为实验组(n=24)、假手术组(n=6)及对照组(n=6)。于接种后第2周行肝动脉、门静脉造影检查,实验组以碘化油栓塞肿瘤供血动脉,假手术组经肝动脉灌注生理盐水2 ml,对照组于造影后处死。实验组、假手术组动物于第3周再接受造影检查并被处死。结果36只实验兔中35只为富血供肿瘤,主要由肝左动脉供血。在肿瘤周边癌组织内3组实验兔VEGF表达阳性率分别为(62.04±19.91)%、(43.38±19.65)%、(43.18±14.01)%,MVD计数分别为39.26±6.80、28.69±5.43、28.71±5.68,实验组与假手术组、对照组比较差异均有统计学意义(P<0.05)。VEGF表达阳性率与MVD计数之间呈明显正相关(r=0.46,P=0.01)。结论TACE术后残癌组织的缺氧状态致VEGF表达及MVD计数较栓塞前明显增高,故加强肿瘤抗血管生成治疗是必要的。Objective To analyze the changes in neovascularization of the survival cancerous tissue after transcatheter arterial chemoembolization（TACE） with lipiodol in rabbits bearing VX2 liver tumor.Methods Thirty-six rabbits model bearing VX2 liver tumor were randomly divided into 3 groups： experimental group（group A,n=24）,pseudo-operative group（group B,n=6） and control group（group C,n=6）.Digital subtraction angiography（DSA） examinations of the hepatic artery and portal veins were performed on 15th day after tumor implantation.Rabbits in group A underwent TACE with lipiodol,in group B were dealt with 0.9% sodium chloride 2 ml,in group C were killed after DSA for histopathologic examination.On 22nd day,the remaining models underwent DSA again and then were killed.Results The tumors were hypervascular and mainly fed by the left hepatic artery in 35 rabbits.In the peripheral cancerous tissues,immunohistochemistry showed that the positive rate of the VEGF expression was（62.04±19.91）%,（43.38±19.65）%,（43.18±14.01）% in three groups,and the MVD counting was 39.26±6.80,28.69±5.43,28.71±5.68,respectively.There were statistical differences among three groups（P〈0.05）.There was positive correlation between the VEGF expression and the MVD counting（r=0.46,P=0.01）.Conclusion VEGF expression and MVD counting increasing caused by the hypoxia condition after TACE in rabbits bearing VX2 liver tumor indicates that it is necessary to strengthen antiangiogenesis therapy for liver tumor.

关 键 词：肝肿瘤 放射学 介入性 栓塞 治疗性 碘化油 血管新生化 病理性 动物实验 

分 类 号：R735.7[医药卫生—肿瘤]